其他检测

We demonstrate here the robustness of our diazonium-ani-line-protein derivatives Biochip. We performed 112 ovalbumin injections each followed by injection of regeneration buffer (Glycine/HCl). After more than one hundred regenerations, the biochip remains active as indicated in the histogram.

SPR imaging system can be used for cell-surface binding detection. The technique is convenient for studing in real tim, label-free cells physically interacting on a surface.

Results demonstrate that monolayer surfaces permit accurate SPR analysis of macromolecular interactions and that the SPRi-PLEX configuration is well suited to a simple recovery protocol that provides rapid sensitive mass spectrometry analysis. Clearly at these levels of surface saturation more than 100 spots could be rapidly visualized and the material recovered for analysis by SELDI even with the manual approach used here.

Ferritin is known to exhibit higher thermal and pH stability than most other proteins and exist stably without showing any drastic change in property with changing pH. This stability can be confirmed using zeta potential.

The isoelectric point of lysozyme (which is a protein) was measured. The value obtained coincides well with the one reported in literature. Lysozyme is a form of enzyme that catalyzes the hydrolysis of polysaccharides in vivo. While in vivo, this protein is denatured by changes in temperature and pH, either varying or losing its liveliness. Measuring its dependence on these two parameters thus provides valuable information for the study of enzymatic reactions.

To confirm the possibility of Zeta potential measurement of smallest particle, Lysozyme has been chosen. Measurement results indicate 2.0 nm particle’s Zeta potential measurement is successfully executed using HORIBA’s SZ-100 nano partica series. In the similar way, Zeta potential of other nano particles including nano proteins can be measured using HORIBA’s nano patrica.

Deoxygenation of proteins is crucial to determination of their intrinsic lifetimes. The HORIBA Jobin Yvon Fluoro-Cube lifetime spectrofluorometer is a sensitive and important tool for investigation of the properties of proteins and changes in these proteins’ microenviroments.

Comparison of the simple triexponential deconvolution with the freeform MEM shows that they are qualitatively similar for HSA-Q binding. The MEM is more sensitive to environmental and structural changes in the complex. The HORIBA Jobin Yvon 5000U lifetime spectrofluorometer with TCSPC is an integral part of researching structure-property relations during pro-tein-binding studies for the biochemical and medical fields.

The authors describe the characteristics and application of a 265 nm AlGaN light-emitting diode LED operated at 1 MHz repetition rate, 1.2 ns pulse duration, 1.32 μW average power, 2.3 mW peak power, and ?12 nm bandwidth. The LED enables the ?uorescence decay of weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH of 6–9.2, the authors ?nd evidence for a biexponential rather than monoexponential decay, providing direct evidence for the presence of phenylalanine rotamers with a photophysics closer to the other two ?uorescent amino acids tryrosine and tryptophan than has previously been reported. © 2006 American Institute of Physics. ?DOI: 10.1063/1.2245441?

We demonstrate an AlGaN light-emitting diode LED giving pulses of ?600 ps full width half maximum, 0.35 ?W average power, 0.6 mW peak power, and ?12 nm bandwidth at 295 nm. This source is ideal for protein intrinsic tryptophan ?uorescence decay research without the unwanted excitation of tyrosine and paves the way to lab-on-a-chip protein assays using ?uorescence decay times. Fluorescence decay and anisotropy decay measurements of human serum albumin are reported and the usefulness of the 295 nm LED demonstrated in comparisons with a nanosecond ?ashlamp and LEDs with nominal wavelength emission of 280 nm. © 2005 American Institute of Physics.[ DOI: 10.1063/1.1984088?]

Most important for you, Jobin Yvon,uniting Spex ® and IBH, now offers both time- and frequency-domai instruments to optimize your research time and effort. The choice is yours.

Fluorescence spectroscopy has demonstrated a way to characterize and quantify skin aging and photoaging. The Fluorolog® allows direct in-vivo monitoring of endogenous skin fluorescence, which makes it possible to follow markers (tryptophan and collagen) in skin, and their changes in both healthy and diseased tissue. It also provides the possibility of evaluating the effect on skin from the modifications of these native proteins or the induction of The Fluorolog® will find a wide range of applications in skin research and photo-dynamic therapy.

Polarization measurements using HORIBA Jobin Yvon spectrofluorometers are a sensitive tool for probing many biochemical interactions.

Two-dimensional velocity fields around a freely swimming freshwater black shark fish in longitudinal (XZ) plane and transverse (YZ) plane are measured using digital particle image velocimetry (DPIV). By transferring momentum to the fluid, fishes generate thrust. Thrust is generated not only by its caudal fin, but also using pectoral and anal fins, the contribution of which depends on the fish’s morphology and swimming movements. These fins also act as roll and pitch stabilizers for the swimming fish. In this paper, studies are performed on the flow induced by fins of freely swimming undulatory carangiform swimming fish (freshwater black shark, L = 26 cm) by an experimental hydrodynamic approach based on quantitative flow visualization technique. We used 2D PIV to visualize water flow pattern in the wake of the caudal, pectoral and anal fins of swimming fish at a speed of 0.5–1.5 times of body length per second. The kinematic analysis and pressure distribution of carangiform fish are presented here. The fish body and fin undulations create circular flow patterns (vortices) that travel along with the body waves and change the flow around its tail to increase the swimming efficiency. The wake of different fins of the swimming fish consists of two counterrotating vortices about the mean path of fish motion. These wakes resemble like reverse von Karman vortex street which is nothing but a thrust-producing wake. The velocity vectors around a C-start (a straight swimming fish bends into C-shape) maneuvering fish are also discussed in this paper. Studying flows around flapping fins will contribute to design of bioinspired propulsors for marine vehicles.

Many marine organisms have complex life histories, having sessile adults and relying on the planktonic larvae for dispersal. Larvae swim and disperse in a complex fluid environment and the effect of ambient flow on larval behavior could in turn impact their survival and transport. However, to date, most studies on larvae–flow interactions have focused on competent larvae near settlement. We examined the importance of flow on early larval stages by studying how local flowand ontogeny influence swimming behavior in pre-competent larval sea urchins, Arbacia punctulata. We exposed larval urchins to grid-stirred turbulence and recorded their behavior at two stages (4- and 6-armed plutei) in three turbulence regimes. Using particle image velocimetry to quantify and subtract local flow, we tested the hypothesis that larvae respond to turbulence by increasing swimming speed, and that the increase varies with ontogeny. Swimming speed increased with turbulence for both 4- and 6-armed larvae, but their responses differed in terms of vertical swimming velocity.

A Fluorolog® spectrofluorometer, equipped with a 450-W xenon lamp, single-grating monochromators, and near-IR accessories (DSS-IGA020L InGaAs detector and emission grating at 600 grooves mm , blaze = 1000 nm) was used to measure the 1O2 emission spectrum. A high-pass filter (λ = 780 nm) acted as an order-sorter, necessary when such large wavelength-ranges are spanned. Fig. 1 shows the emission spectrum with an integration time of 0.1 s and a bandpass of 12 nm. Note the excellent signal-to-noise ratio.

Besides providing insight into mechanisms of photosynthesis and a possible monitor of reef stability through bleaching, these spectra act as a catalog of coral identity to help distinguish between specific coral types. ?

Polarization measurements using HORIBA Jobin Yvon spectrofluorometers are a sensitive tool for probing many biochemical interactions.

Polarization measurements using HORIBA Jobin Yvon spectrofluorometers are a sensitive tool for probing many biochemical interactions.

The ability to monitor kinetic process occurring on the millisecond timescale has been demonstrated by the simple addition of a stopped flow accessory to the DeltaFlex system. This was operated in the kinetic TCSPC measurement mode and was aided by the use of a high repetition rate excitation source coupled with very low dead time electronics to efficiency collect the data. The data obtained clearly shows the advantage of using the fluorescence lifetime in this case, to obtain obtain kinetics unaffected by sample photobleaching.

Raman microscopy has been used to interrogate single bacteria cells – with multivariate analysis it has been shown that different species and growth phases can be quickly distinguished. Cells grown with differing amounts of 13C enriched nutrients showed Raman bands shifted to lower wavenumber due to the heavy isotope. With further multivariate analysis the cells can be classified according to the percentage uptake of 13C.

It has been shown that the analyte rhodamine 6G can be successfully measured and identified within single living cells using surface enhanced Raman microscopy. Principal components analysis for covariant features in the spectra allowed the identification of rhodamine 6G despite extensive spectral overlap from the cellular matrix.

This application note illustrates the suitability of the UVISEL Spectroscopic Phase Modulated Ellipsometer as a very accurate instrument for studying solid/solid or solid/liquid interface reactions, and measurement of biofilm thickness.

This interpretation has been supported by both surface tension measurement for the liquid phase and fluorescence spectroscopy for bulk behaviour. From this study there is clear evidence that increasing the substitution rate from 10% to 35% induces a reinforcement of the hydrophobic interactions and thus the formation of more compact and stable aggregates which can complex hydrophobic compounds. The use of CMP 35% C8 could be envisaged in drug formulations where dispersion of a hydrophobic drug in an aqueous phase is required.

The sample data is summarised in the figure. The thickness of the DNA sensor pad was found to be of the order of 20 ?. This is consistent with the preparation of a layer of single molecular thickness as expected. The pads characterised had a variety of shapes. The example chosen here had a ring shape. The area covered by the analysis is 50 mm x 20 mm.

瑞典百欧林科技有限公司是一家先进科研仪器生产商，在北欧的瑞典，丹麦和芬兰都有主要产品的研发和生产基地。我们为用户提供高科技、高精度的科研设备，可用于表界面、材料科学、生物科学、药物开发与诊断等研究领域。

ViroCyt通过技术创新，在数分钟内直接、特异性地进行病毒定量，远远胜于耗时、低精度的噬斑法、TCID50等传统方法。利用荧光标记的高亲和力抗体，特异性识别目标病毒的特定抗原表位，既可以提高检测精度，也可以达到混合病毒中目的病毒鉴定的功能。

可在不稀释蛋白质配方的情况下检测蛋白质的稳定性； 蛋白质的变性或稳定性取决于温度和时间，根据温度变性可以立即发生或有明显的延后期； VROC? 可以检测到展开或变性造成的粘度的微小增加

特性粘度是指添加极小量溶质到溶剂后引起的粘度的增加，有必要通过测量不同浓度下的溶液粘度以测定特性粘度，特性粘度是在零浓度下定义的值： 是溶液与溶剂的相对粘度。 Eq. (1)中 C 是浓度，单位是 mg/ml 或 mg/dl. 证实m-VROC?可通过检测特性粘度以测定蛋白质，聚合物和其它大分子的分子大小

PSI公司是捷克科学院Nedbal教授与现任PSI公司总裁、布尔诺（孟德尔发现遗传定律的城市）植物表型研究中心主任Trtilek博士共同成立的，是真正顶尖植物科学家建立的公司，本身就承接大量欧盟的科研项目并与捷克科学院联合成立了捷克全球变化中心。