铁蛋白中Zeta电位检测方案(激光粒度仪)

ADS-SZ-ZE-012nanoparticaNANO PARTICLE ANALYZERSZ-100Nano Particle AnalyzerApplications Data Sheet .Zeta Potential Measurement of Ferritin Ferritin is a protein which is abundant invarious parts of the human body， includingliver， spleen， bone marrow， and muscle； it isknown as an iron-binding protein associatedwith the storage of iron ions (Fe3+). Whenone suffers from anemia， a disease causedby iron failure in the body， ferritin levels firstdecrease followed by decreases in serumiron and hemoglobin levels. This protein isalso a promising cancer marker. Among thedevice-fabrication processes which haverecently become matters of concern is thefabrication process of novel nanostructuresby bottom-up-type nanotechnology， knownas a bio-nanoprocess， which involves theuse of bio-mineralization the phenomenonof aggregation-based crystal growth ofinorganic substances contained in nucleicacids and proteins. Method Analyzer： SZ-100 Measurement Temperature： 25.0 degree CSample： Ferritin Results The measurement result offferritinnanoparticles is shown in Fig. 2. The zetapotential of this sample was -35.0 mV.There appears in the graph a measurementof the zeta potential of the sample in aTris-Hcl buffer’ solution.*Theferritinmeasured in this experiment has nothing todo with the picture of Fig. 1. This process utilizes the phenomenon ofaggregation-based crystal9growth ofinorganic substances contained in ferritinand other bio-molecules (bio-mineralization)1) Fig. 1 Ferritin， a basket protein' Source： Provided through the courtesy ofPanasonic Corporation Disperse medium： Tris-Hcl buffer solution(approximately pH 7.8-8.8)Refractive index of disperse medium： 1.333 Conclusion Ferritin is known to exhibit higher thermal and pH stability than most other proteins and existstably without showing any drastic change in property with changing pH. This stability can beconfirmed using zeta potential. * The information described here has no relevance to Panasonic Corporation. ))MPanasonic Technical Journal Vol.54 No.3 2008 Explore the future HORIBAC HORIBA， Ltd. All rights reserved. Ferritin is known to exhibit higher thermal and pH stability than most other proteins and exist stably without showing any drastic change in property with changing pH. This stability can be confirmed using zeta potential.