酶、非酶分子中和频光谱检测方案(其它光谱仪)

      The content of this thesis is an investigation into physicochemical, photophysical,and interfacial properties of β-galactosidase, a crucial enzyme, its conjugate with CDs,interaction of β-galactosidase with X-gal, and surface chemistry and spectroscopic studyof toxins, Cholera toxin B (CTB) and Fumonisin B1 (FB1). The objective of the work wasto understand the surface chemistry behavior of the important enzyme, conjugates andtoxins in a model systems representing vaguely cell membrane environments, to prove theactivity of the deposited enzyme, its conjugate, and toxins in a form of the monolayer. Wealso studied the effect of green LED light on the FB1 Langmuir monolayer which will beuseful for possible future treatment of fungal keratitis without any side effects. Furthermore, this approach can be employed to understand the property of transmembrane receptors, to study the cell adhesion, and its implications. Besides that, this information is relevant for the successful deposition of Langmuir-Blodgett (LB) film. The Langmuir monolayer isotherm of enzyme, conjugate and the toxin were studied using the surface chemistry and spectroscopy at the air-water/subphase interphase.      We employed the pressure (π)-area (A) isotherm measurement which is typicallythe very first step in the study of these enzymatic and non-enzymatic molecules floating atthe subphase surface as it gives useful information on the existence of different phases,phase transitions and the stability of Langmuir monolayer at fixed temperature and pH.       During our study we found that β-galactosidase as well as its conjugate form stableLangmuir monolayer. We observed that during the interaction of the enzyme with X-gal,its secondary structure changes. This does not mean that the overall structure of the enzymegets changed. This is because enzyme structure is determined by primary, secondary,tertiary, and quaternary structure. The interfacial study of CTB showed that the moleculeis very stable at the air-subphase interface. In performing surface chemistry andspectroscopic study of FB1 molecule, the interesting finding that came out of the FB1Langmuir monolayer investigation was that the analyte adopted stability (withoutirradiation of green LED light) at the monolayer and remained degraded with subsequenteffect of the green LED light (525 nm) at air-water interface.