细胞,生物医学样品中 成像检测方案(激光拉曼光谱)

In this work multimodal operation of commercially available CARS microspectrometer (EKSPLA CARSCOPE) equipped with a picosecond dual wavelength laser source (EKSPLA PT259) was demonstrated for the imaging of various biological objects using coherent anti‑Stokes Raman scattering (CARS), two photon excited fluorescence (TPEF) and second harmonic generation (SHG) contrast mechanisms. Obtained CARS‑images of yeast cell and flower pollen clearly demonstrate label‑free and 3D imaging capabilities of CARS technique. Multi‑contrast images of green algae and plants containing starch demonstrate a variety of research facilities, where the method can be applied.