饲料中粗脂肪检测方案(抽提萃取)

APPLICATION NOTEF&F-S-002-2019/A1 CRUDE FAT DETERMINATION IN FEEDRANDALLMETHOD Crude Fat Determination in Feedaccording to the Randall method Reference： ISO 11085：2015 Cereals， cereals-based products and animal feeding stuffs--Determination of crude fat and total fatcontent by the Randall extraction method Tested with VELP Scientifica SER 158/6 Solvent AutoExtractor (Code S303A0380) ( 名称：北京盈盛恒泰科技有限责任公司 ) E-mail： sales@ensoultech.com Introduction Fat is an important nutrient in feed rations for cattle， pig， poultry， sheep， horse and pet foods， as a high energy feedingredient. Fats and oils， contain about 2.25 times as much digestible energy as the carbohydrates in grain. They are veryconcentrated sources of energy when added to animal feeds to increase the energy density of the ration. Adding fats andoils will reduce the dustiness of feeds， and reduce ‘fines’ in pelleted diets， adding desirable characteristics which havevalue. Fats and oils can also improve a ration by improving palatability. In particular， cow nutrition is dependent uponadequate energy， protein， vitamins and minerals in a balanced diet， but research is showing that fat content in a cow’sdiet can enhance rebreeding success. The results here presented obtained with VELP SER158 are part of the proficiency testing program organized byBIPEA. The SER 158 results have been compared with the BIPEA expected results and were fully in line with BIPEAtolerance range. Crude Fat determination in feed Hot solvent extraction process with SER 158 Series can be summed up in 5 steps， for a fully unattended operation： STEP 5 COOLING During IMMERSION the sample is immersed in boiling solvent. Then the REMOVING step automatically lowers the levelof the solvent to below the extraction thimble.During WASHING the condensed solvent flows over the sample and throughthe thimble to complete the extraction process. The fourth step involves solvent RECOVERY. Approximately 90%of thesolvent used is collected in the internal recovery tank. The final step is the COOLING of the extraction cups containing theextracted matter. The cups are raised to prevent burning. The extraction cups containing the extract are placed in a dryingoven， cooled in a desiccator and weighed for the extract percentage calculation. Extraction thimbles (33x80 mm) (Code A00000295) Viton seals Glass extraction cups (Code A00000290) ●FPetroleum Ether 40-60 °C as solvent Extraction Procedure with SER 158 Select“Analysis” on the ControlPad and then method"Crude fat in feed" including the following parameters： ● lmmersion Time： 25 minutes Recovery Time 30 minutes eRemoving Time： 10 minutesCooling Time： 4 minutes Washing Time：55 minutes Petroleum Ether 40-60°C， 100 ml Close the safety guard and add the solvent using the automatic solvent dispensing system SolventXpressTM to minimize exposure to the solvent ensuring operator safety.Press START to begin the extraction process. At the end of analysis position the extraction cups containing the extract ina drying oven (1 hour at 105°C)， cooled them in a desiccator to room temperature. In Results menu select the extractioncups batch ID analyzed， press calculate， scan the extraction cups with barcode reader and weigh (Total). Results on BIPEA Feed samples Analysis results are calculated automatically and stored in the ControlPad when entering the weights into the software(manually or automatically through a balance). The extract percentage calculation is performed by using the following formulas： Where： Sample= sample weight (g) Tare= weight of the empty extraction cup (g) Total =weight of the extraction cup + extract (g) Sample name Tare (g) Sample (g) Total (g) Extract (g) Extract (%) Feed for piglet 134.1359 3.0308 134.3363 0.2004 6.61 133.5234 3.0330 133.7269 0.2035 6.71 133.8042 3.0263 134.0079 0.2037 6.73 Average ±SD% 6.68±0.06 RSD%* 0.95 Fish meal 133.8927 3.0105 134.1009 0.2082 6.92 133.6175 3.0197 133.8273 0.2098 6.95 132.9321 3.0292 133.1441 0.2120 7.00 Average ± SD% 6.95±0.04 RSD%* 0.60 Laying hen feed 134.1314 3.0095 134.2337 0.1023 3.40 132.9316 3.0157 133.0325 0.1009 3.35 138.5382 3.0684 138.6420 0.1038 3.38 Average± SD% 3.38±0.03 RSD%* 0.81 ( 名称：北京盈盛恒泰科技有限责任公司 ) ( E-mail： sales@ensoultech.com ) Turkey feed 135.7200 3.0031 135.8093 0.0893 2.97 138.1092 3.0113 138.1994 0.0902 3.00 140.0642 3.0085 140.1552 0.0910 3.02 Average ± SD% 3.00±0.03 RSD%* 0.86 *RSD%=(Standard Deviation x 100)/ Average Conclusion The results obtained are reliable and reproducible in accordance with the expected values， with a low relative standarddeviation (RSD<1%)， that means high repeatability of the results. Therefore， SER 158 Solvent AutoExtractor is ideal forthe crude fat determination in feed in accordance with ISO 11085：2015 Method. Benefits of hot solvent extraction (Randall) by using SER158 Automatic Solvent Extractor： up to 5 times faster than Soxhlet (hot solvent vs. cold solvent) ● low solvent consumption (high solvent recovery， approximately 90%)-limited cost per analysis ● no exposure to solvent worldwide official method full traceability with automatic result calculation and on-board archive Cloud connectivity to VELP Ermes ensuring software updates， real time monitoring and control， notificationsand enhanced service support. ( 名称：北京盈盛恒泰科技有限责任公司 ) 地址：北京市西城区广安门外大街朗琴国际大厦B座电话：址：www.ensoultech.com       脂肪是牛、猪、家禽、羊、马和宠物饲料中重要的营养成分，是一种高能量饲料成分。脂肪和油类所含的可消化能量是谷物中的碳水化合物的2.25倍。当添加到动物饲料中以增加口粮的能量密度时，它们是非常集中的能量来源。添加脂肪和油脂将减少饲料的灰尘，减少颗粒饲料中的细粒，增加有价值的理想特性。脂肪和油也可以改善口粮的口感。特别是，奶牛的营养依赖于均衡饮食中充足的能量、蛋白质、维生素和矿物质，但研究表明，奶牛饮食中的脂肪含量可以提高育种的成功率。实验样品：Feed for piglet    ID: 12-1513-0207 Crude fat assigned value: 6.7 % Tolerance range: 6.1–7.3 %Fish meal             ID: 10-0313-0190 Crude fat assigned value: 6.9 % Tolerance range: 6.3–7.5 %Laying hen feed  ID: 15-2413-0052 Crude fat assigned value: 3.4 % Tolerance range: 2.8–4.0 %Turkey feed         ID: 10-3613-0231 Crude fat assigned value: 2.8 % Tolerance range: 2.2–3.4 %实验仪器：SER158自动脂肪测定仪（意大利VELP）结果分析：所得结果与期望值一致，重复性好，相对标准偏差低(RSD < 1%)，重复性好。因此，根据ISO 11085:2015方法，SER 158自动脂肪测定仪是饲料中粗脂肪测定的理想选择。 使用SER158自动脂肪测定仪(Randall)的好处:►5倍索格利特(溶剂热比冷溶剂)►溶剂消耗低(高溶剂回收,大约90%)——有限的成本分析►没有暴露在溶剂►标准的方法►完整的可追溯性和自动计算结果和车载存档►云连接VELP erm确保软件更新,实时监测和控制,通知和增强服务支持。