粗纤维含量与饲料营养价值有密切关系,粗纤维含量越高,营养价值越低。纤维是饲料中需要确定的一个重要参数。通过参加BIPEA组织的能力验证项目,对VELP FIWE全自动纤维素测定仪性能进行了评估。采用先进的纤维分析仪对样品进行了分析,并与豌豆的耐受范围进行了比较。
APPLICATION NOTEF&F-F-001-2019/A1 ADF DETERMINATION IN FEED ADF Determination in Feed Acid Detergent Fiber (Van Soest method) Reference: ISO 13906:2008, Animal feeding stuffs - Determination of Acid Detergent Fiber (ADF) AOAC 973.18 Fiber (Acid Detergent) in Animal Feed Tested with VELP Scientifica FIWE Advance Fiber AutoExtractor (Code F30500500) and COEX Cold Extractor(Code F30520204) 名称:北京盈盛恒泰科技有限责任公司 地址:北京市西城区广安门外大街168号朗琴国际大厦B座603室电话:010-83993592网址:www.ensoultech.com ( E-mail: sales@ensoultech.com ) Introduction The single most important determinant of forage quality is stage of maturity of the plant when harvested. Plants, likeanimals, grow and mature over time. The mature plant is one that has developed reproductive components to the point ofgenerating seeds. Immature forage is the lush rapidly growing plant prior to reproductive parts (seeds, flowers)development. Mature plants contain greater amounts of cell wall structural components, and lignin for cell wallreinforcement. These cell wall components allow the large mature plant to stand upright, rather than to fall over under its own weight.This increase in lignin and fiber results in a dilution of energy, protein and other nutrients as well as a decline in nutrientdigestibility. There is a close relationship between the crude fiber content and the nutrition value of the feed: the higher the crude fiberis, the lower the nutritional value is. Fiber is thus an important parameter to be determined in feed. The performance of VELP FIWE Advance Fiber AutoExtractor was evaluated by participating in the proficiency testingprogram organized by BIPEA. Samples were analyzed using FIWE Advance Fiber analyzer and the obtained resultswere compared with the BIPEA tolerance range. Cell Content Cell Wall Crude Protein NDF Crude Fat Organic rest Sugar ADF Starch ADL Hemicellulose Pectins IHemicellulose Cellulose Cellulose Lignin Lignir Cellulose Lignin Lignin Determination of Acid Detergent Fiber content in different feed samplesThe ADF determination can be summed up in 3 steps: DIGESTION ·Filtering DEFATTING · Boiling ·Washing with *Acetone *performed with COEX During BOILING & FILTERING (DIGESTION) the Acid detergent solution (ADS) solubilizes the hemicellulose while ligninand cellulose remain insoluble into the crucible. During WASHING the residues into the crucibles are washed with water to remove detergent residues. The final step isthe DEFATTING of the samples where the last washes are performed with acetone. BIPEA Samples Laying hen feed ID: 15-2413-0052 ADF assigned value: 5.8 % Tolerance range: 4.3-7.3% Fattening feed for cattle ID: 1-4213-2018 ADF assigned value: 10.1% Tolerance range:8.6-11.6 % Chemicals and Equipment required ● Analytical balance, 4 decimals . Glass Crucibles P2, 6pcs (A00000140) . Acid Detergent Solution ADS. n-octanol as antifoaming agent Acetone, technical grade Crucibles Preparation Dry the crucibles in an oven at 102-105 °℃ for 2 h to 4 h and cool them in a dessicator. Connect the optional VELP barcode reader(Barcode scanner with USB socket Code: A00000364 or Wireless barcode scanner Code: A00000365) to the FIWE Advance.Select Analysis/Details, scan the crucible and weigh the tare (Mtare) to the nearest 0.0001 g. Then weigh 1 g of sample portioninto each crucible (Msample) and transfer the values from the balance to FIWE Advance. This operation is repeated sequentiallyfor the remaining positions. Include two blanks for the first 20 to 30 samples and add one blank for each additional 20 to 30 samples (Btare). Analysis with FIWE Advance Select“Analysis”and standard method “ADF -Acid detergent fiber (Van Soest)" including the following parameters: · Crucibles porosity P2·Enzyme:No● Preheat: No·Digestion time: 60 minutes· ADS: 100 ml·Washing: 3x 50 ml ml of distilled water●N-octanol:Yes Lower the lever and position the heating shield. Press START to begin the process. At the end of analysis remove the crucibles from the unit, place them in COEX unit fordefatting (25 ml acetone for 3 times) and dry them (130℃±2°C for 2 h). Leave to cool in the desiccator. In Results menuselect the crucibles batch ID analyzed, press calculate, scan the crucibles with barcode reader and weigh to the nearest0,0001 g (Mdry and Bdry). 名称:北京盈盛恒泰科技有限责任公司 地址:北京市西城区广安门外大街168号朗琴国际大厦B座603室电话:010-83993592网址:www.ensoultech.comE-mail:sales@ensoultech.com Results on feed samples Mdry = sample weight after drying Mtare = tare ofthe sample Msample = sample weight Bdry =blank weight after drying Btare = tare of the blank Sample Mtare (g) Msample (g) Mdry (g) ADF% Laying hen feed 31.6282 1.0021 31.6797 5.2590 31.3739 1.0183 31.4328 5.2931 31.6810 1.0364 31.7399 5.2007 Average± SD% 5.25±0.05 RSD%* 0.9 Fattening feed forcattle 29.4891 1.0081 29.5990 11.0207 30.1385 1.0051 30.2468 10.8944 30.2660 1.0016 30.3729 10.7927 Average±SD% 10.90±0.11 RSD%* 1.0 * RSD%= (Standard Deviation * 100) / Average ADF Blank (Bdry-Btare) results: -0.0012 g Conclusion The obtained results are reliable and in accordance with the expected values, with a low relative standard deviation(RSD≤1%), that means high repeatability of the results.The use of an extraction apparatus purposely devised for thismethod as FIWE Advance unit, makes very easy the standardization of analytical conditions. Benefits of FIWE Advance are: 6 positions simultaneously and unsupervised Easy to use: 7"touch screen operation with preset method and favorite methods setting Automatic heating and dispensing of reagents avoiding any possible contact with chemicals and fumes ● State-of-the-art safety features controlling all the steps of the analysis Precision and accuracy: high reproducibility of the results: ± 1% relative or better Results in accordance with official procedures Connection to VELP Ermes cloud platform to monitor and control the instrument and to access to your database. In order to avoid losses of fiber, it’s important to remember that crucibles life is around 20-30 analysis, because the fritted filter could bedamaged from basic and acid solutions. Hence it’s suggested to change them after 20-30 analysis. 名称:北京盈盛恒泰科技有限责任公司 名称:北京盈盛恒泰科技有限责任公司地址:北京市西城区广安门外大街朗琴国际大厦B座电话:址:www.ensoultech.comE-mail: sales@ensoultech.com 牧草质量的一个最重要的决定因素是收获时植物的成熟期。植物像动物一样,随着时间的推移而成长和成熟。成熟的植物是已经发育出生殖成分到能产生种子的植物。未成熟牧草是指植物在生殖部分(种子、花)发育前繁茂快速生长的牧草。成熟的植物含有更多的细胞壁结构成分和木质素加强细胞壁。这些细胞壁成分使大型成熟植物直立,而不是被自己的重量压垮。 粗纤维含量与饲料营养价值有密切关系,粗纤维含量越高,营养价值越低。纤维是饲料中需要确定的一个重要参数。通过参加BIPEA组织的能力验证项目,对VELP FIWE全自动纤维素测定仪性能进行了评估。采用先进的纤维分析仪对样品进行了分析,并与豌豆的耐受范围进行了比较。实验样品:Laying hen feed ID: 15-2413-0052 ADF assigned value: 5.8 % Tolerance range: 4.3 – 7.3 %Fattening feed for cattle ID: 1-4213-2018 ADF assigned value: 10.1 % Tolerance range: 8.6 – 11.6 % 实验结果: 所得结果可靠,符合期望值,相对标准差低(RSD≤1%),结果重复性好。使用为本方法设计的萃取装置作为先进单元,使分析条件的标准化变得非常容易。FIWE Advance的好处是:•6个位置同时进行,无人监督•使用方便:7”触摸屏操作,预置方法和选用的方法设置•自动加热和分配试剂,避免任何可能接触化学品和烟雾•先进的安全功能控制分析的所有步骤•精密度和准确度:结果重现性高,相对或更好•结果符合标准程序•连接VELP Ermes云平台,监控仪器和访问您的数据库。
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北京盈盛恒泰科技有限责任公司为您提供《饲料中酸性洗涤纤维检测方案(纤维测定仪)》,该方案主要用于饲料中理化分析检测,参考标准--,《饲料中酸性洗涤纤维检测方案(纤维测定仪)》用到的仪器有FIWE ADVANCE 全自动纤维素测定仪、意大利VELP公司-纤维素测定仪