其他检测

激光剥蚀ICP-MS是用于检测、量化并对生物组织切片上的金属离子分布进行成像的最实用、灵敏度高的一种检测技术。而且，随着单克隆抗体的重金属标记的应用发展，它可以同时用于测量蛋白的分布。这项技术上基于组织上的多线扫描的定位。

BTC 根系动态观测系统采用微根管（Minirhizotron，又称微根窗）技术，这是一种非破 坏性、定点直接观察和研究植物根系的方法，其最大优点是在不干扰细根生长过程的前提下， 可以用来连续跟踪监测细根从出生到死亡的生长过程，也能记录下细根乃至根毛和菌根的生 产和物候等特征，揭示土壤环境下的生态系统多样性。采用微根窗技术是估计生态系统地下 C 分配和 N 平衡研究的有效方 法，对于研究植物根系的动态 生长变化，细根的周转速率， 评价植物碳汇贡献有着十分重 要的意义。

从采集的久居陕南地区居民离体牙中挑选富有代表性的牙齿30颗。按照李子夏等人[14]实验方法采用加热敲击法将牙齿的牙釉质和牙本质分开，收集分开的牙釉质和牙本质，用玛瑙钵研磨至0.075 mm。准确称取研磨后样品0.05 g于微波消解罐中，加5 mL50%的HNO3和2 mL 30%的H2O2溶液，轻轻晃动消解罐，然后滴入约2 mL Milli-Q水，在1000 W的功率下进行微波消解。微波消解升温程序为：5 min内由室温升到120 ℃，并在此温度保持3 min，然后在8 min内继续升温到220℃，并在此温度下保持15 min 至消解结束。试样消化完全后，自然冷却。用Milli-Q水转移至25 mL容量瓶中，定容。同步做试剂空白实验。实验中用骨头标准样品对消解过程进行质量控制。

SPEX 2010高通量动植物组织研磨机可以同时高效均质576个样品，一般样品的处理时间只需2分钟，大大提高了样品处理效率，而且样品处理间的重复性非常好。

使用SPEX Freezer Mill 冷冻研磨机研磨，可高效研磨骨头等坚硬材料，并确保分析材料一直处于零下196℃，保存被分析材料的生物活性不被破坏.

Stabino是一个不需要样品参数信息就可以做滴定的有效的工具。该仪器适合用于筛选工作。蛋白质溶解度对pH 的依赖是影响稳定的蛋白质溶液形成的一个关键因素。 蛋白质的溶解度取决于pH 值、盐浓度、离子类型、温度和溶剂。蛋白质分子如溶菌酶、胰岛素或白蛋白有含离子侧基团的聚电解质特征。在等电点(IEP)蛋白质溶解度是零。因此，会发生聚合。

高分辨率图像，全自动化操作，15 秒抽真空，30 秒成像，光学导航结合自动马达样品台，实现点到哪里看到哪里。

Coxem台式电镜可采用低真空成像技术以及选配冷台等附件，实现样品的高分辨观察。COXEM台式扫描电镜应用已经深入到很多方面。

凝胶色谱柱(GPC) 具有自动化程度高、可有效去除样品中大分子物质( 如蛋白质、脂肪等干扰物质) 等优点，常被用来与固相萃取柱(C18 或HLB 萃取柱) 或填充硅胶柱串联使用，从而达到样品净化的目的 。GPC应用虽已较为广泛，但针对其净化和加标回收49 种 BFＲs 类 化合物的资料却少有报道。

The IMV-4000 enables infrared imaging by a figure of two or more faster than using a conventional single element detector. A technique that quickly analyzes the results is also desirable. JASCO has proposed an analysis of imaging data with a variety of multivariate analysis techniques to accelerate the processing of infrared imaging data. For example, we have made it possible to easily create a visualization of the distribution map for the various components of multilayer film by analyzing imaging data using principle component analysis (PCA). We have also created a model that performs secondary structure estimation (SSE) on proteins based on IR spectra by principal components regression (PCR) and

Terpenes in essential oils are easily oxidized and can cause a decrease in the flavor in foods. Removal of Terpenes is commonly practiced in perfume industry to yield higher quality oils. Natural spearmint oilcontains l-limonene that is quite unlike d-limonene, the aroma is not reminiscent of citrus but of a light, clean Terpene hydrocarbon fragrance. Conventionally, Terpenes are removed using vacuum distillation. However, it is difficult to monitor the fractionation process in real-time during this process. We used SFC with a PDA detector and an FTIR spectrometer as a dual monitoring system to enable selective monitoring of the target compound Carvone in real time during elution and fractionation. The PDA provides broad information on the separation, but cannot differentiate between limonene and Carvone.

It is generally understood that chiral compounds have different bioactivities depending upon the absolute configuration of each compound. Some familiar examples include glutamic acid and thalidomide. Lglutamic acid demonstrates the “Umami” taste*1, while D-glutamic acid has a bitter taste, similarly, the R form of thalidomide is a sedative, but the S form has teratogenic activity. Thus, the separation and study of chiral compounds is critical for many reasons. The functionalities of chiral compounds have been studied for the development of advanced molecules for many applications. The study of chiral compounds has spread to several fields such as natural products, pharmaceuticals and other functional molecules, and it can be pointed out that among those studies, the structural analysis of chiral compounds is a very important topic. X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR) and Electronic Circular Dichroism (ECD) using UV/Vis light are employed as primary methods for the structural analysis of chiral compounds. In this paper, the measurement of chiral compounds by Vibrational Circular Dichroism (VCD) using infrared light will be outlined. VCD is a method to measure the difference of absorbance intensity between left-hand and right-hand circularly polarized light as shown in Figure 1. It is an advantage of VCD that this method can be applied to almost all organic compounds in the same way as infrared (IR) spectroscopy. In addition, by comparing the measurement results with calculated results by ab-initio molecular orbital calculations, the absolute configuration of the sample can be determined. However, since the peak intensity of VCD spectra are 1,000 – 10,000 times weaker than that of standard IR spectra, spectroscopic instruments with high sensitivity and stability with very small baseline fluctuations are required. The FVS-6000 VCD system has a high sensitivity detector, suitable optical

Introduction CD spectra provide information on the secondary structure of proteins and the environment of aromatic side chains. Therefore, CD measurement using a stopped-flow system is considered as one of the best methods for analyzing the unfolding and refolding of proteins. The existence of an intermediate between denaturated state and natural state during the refolding of proteins has been reported. The CD stopped-flow method is used for examining this refolding process. In this report the refolding process of cytochrome c (cyt c) measured using a SFS-492 stopped-flow system will be explained. Keywords: Stopped-flow, Circular Dichroism, Refolding Sample Preparation Aqueous solution of Cytochrome c denaturated by guanidine hydrochloride (GuHCl) was diluted with 0.1 M acetic acid buffer solution (1:9). The refolding process was observed at 222 nm for the secondary

Amphetamines are controlled substances which are potent stimulants that induce feelings of strength and energy while decreasing the need to sleep or eat. The majority of illegal amphetamine products are racemic; consequently, both isomers will appear in the biological fluids unchanged. In comparison the break-down products formed by the metabolism of most pharmaceutical drugs show the appearance of pure amphetamine enantiomers. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of right- and leftcircularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. Since different enantiomers absorb different portions of the light, it provides and excellent means for enantiomer detection. In this application CD will be used to study the isomers of amphetamine and methamphetamine. Introduction Amphetamines are synthetic drugs that stimulate the

The Stopped Flow method is used to analyze reactions occurred during the duration of several milliseconds and several seconds meanwhile the CD measurement provides valuable information regarding the protein’s secondary structures and environment of aromatic amino acids. Therefore, CD measurement with Stoppedflow method provides one of the best techniques in analyzing the protein’s Unfolding and Refolding process. Concanavalin A (derived from Jack bean) in natural state has abundant β-sheet structures, however, it is known that its structure changes into the one with rich α-helix under trifluoroethanol (TFE) and its Unfolding process has been reported also. In this application, the model SFS-492 High-Speed Stopped-flow System was used to measure the Unfoldin

Introduction The multivariate SSE program can be used to analyze sets of CD data. A large number of samples can be automatically measured and efficiently analyzed using the high-throughput circular dichroism (HDX) system and multivariate SSE program. Keywords: Secondary structure estimation, Multiple samples, HTCD, HDX Sequence Optical constants are automatically calculated in the multivariate SSE program after imputing parameters

Proteins, biological polymers composed of amino acids, have primary structures indicating the sequence of amino acids and higher three-dimensional order structures as well. The structure of proteins defines their biological functionality and CD spectroscopy is commonly used in the study of proteins and oligopeptide applications. Using secondary structure estimation (SSE) software, the helix, sheet and random coil contents of unknown proteins can be quantitatively estimated from CD spectra. SSE software has been applied mainly in academic or research studies of protein structure. JASCO now offers the new SSE Program based on the PLS/PCR method of multivariate analysis, to cope with expanding usage of protein studies across a wide range of fields, su

Introduction Generally, a sample for CD measurement needs to be in a liquid state to allow transmission measurement, more recently there has been increasing requirement for CD measurement of samples that are insoluble or may change structure in solution. These types of sample are best measured in solid state. Although CD measurement can be made in transmission for solid samples, there is a requirement for sample preparation; such as making a pellet and if sample dilution is required, sample recovery can be difficult. As an alternative, measurement using Diffuse Reflection (DR) CD is recommended. 1), 2) This application note illustrates the use of DRCD measurement with the DRCD-575 diffuse reflectance accessory (See Fig. 1.) optimized for the J-1500 CD spectrometer with the multi-probe simultaneous CD/LD measurement. DRCD uses incident light to illuminate a solid sample and

细胞的迁移运动不仅是细胞进行很多重要生理活动的基础，同时也是炎症反应和肿瘤发生等病理过程中的重要步骤和关键环 节。细胞迁移 (cell migration) 也称为细胞爬行、细胞移动或细胞运动，是指细胞在接收到迁移信号或感受到某些物质的梯度后而 产生的移动。细胞迁移为细胞头部伪足的延伸、新的黏附建立、细胞体尾部收缩在时空上的交替过程。细胞迁移是正常细胞的基 本功能之一，是机体正常生长发育的生理过程，也是活细胞普遍存在的一种运动形式。胚胎发育、血管生成、伤口愈合、免疫反 应、炎症反应、动脉粥样硬化、癌症转移等过程中都涉及细胞迁移。 目前人们对恶性肿瘤的研究是多方面的，从癌症的产生到转移，血管供给以及分裂增殖都一直是医学和生物学研究的热点。 恶性肿瘤与良性肿瘤的最大区别就在于是否可以对周遭组织进行浸润，结果是形成边界不分明的癌组织，或是进入血管转移到远 端（见远端转移）。根据观察可将癌症浸润分为四步。首先癌细胞表面的粘附分子会减少，与周围的细胞彼此分离，被“解除束 缚”。同时癌细胞与基底膜的粘附却会增加，这是癌细胞通过增加自身基底面层粘连蛋白（laminin）受体实现的。然后癌细胞会 释放蛋白酶，用以降解细胞外基质成分，如Ⅳ型胶原酶，使基底膜受损，产生缝隙。最后是癌细胞阿米巴运动样的迁移，钻过基 底膜的缝隙，到达底下的间质组织。癌细胞此后会继续用蛋白酶为自己在间质组织开路，直到血管，然后它会以同样方式进入血 管，经血到转移后，又以同样方式出管。因此，癌症的形成与肿瘤细胞的迁移能力密不可分，这也成为了一个诊断和治疗癌症的 一个靶点。 研究细胞迁移的方法有很多，比如划痕法、实时细胞追踪、Transwell和细胞芯片等，本文将一一介绍现今比较主流的与细 胞迁移相关的实验方法。

众所周知，单克隆抗体在科研、疾病诊断以及治疗等方面具有非常重要的作用。单克隆抗体药物具有靶向性明确、低毒性和 不良反应少等优点，在肿瘤的生物治疗及靶向治疗中发挥重要作用。目前，抗体药物市场前景广阔，已成为制药行业中发展最 快、活力最强和技术含量最高的领域。为了获得具有特异性的抗体，首先需要筛选能够表达分泌特异性抗体的杂交瘤细胞系。实 验室常规的方法就是通过小鼠脾细胞和骨髓瘤细胞的融合，获得杂交瘤细胞。通过在培养基里加入筛选压力，只有脾细胞和瘤细 胞成功融合的杂交瘤能够存活。筛选杂交瘤的主要挑战在于如何快速、高效地从成千上万的杂交瘤细胞里面找到生长良好、能够 分泌特异性抗体的克隆，并且尽可能确保为单克隆。 Molecular Devices为杂交瘤的筛选提供了全方位的高通量解决方案，能够最大化提高筛选特异性杂交瘤的效率，并且节省 筛选时间。ClonePix2是一个哺乳动物细胞筛选系统，通过在培养基中加入荧光标记检测试剂，能够通过荧光筛选并自动化挑取 表达分泌特异性抗体的杂交瘤克隆。CloneSelect Imager是一个无标记活细胞成像系统，能够客观、快速检测细胞在孔板里的生 长情况，并提供克隆单细胞来源证明，确保筛选稳定的单克隆用于下游进一步研究。此外，为了优化细胞的生长，Molecular Devices提供了CloneMedia和CloneMatrix两种型号半固体培养基用于支持杂交瘤细胞生长形成独立单个的克隆。

Although membrane proteins are very difficult to work with, ultra-sensitive calorimetry can overcome the limitations of many other physical characterization techniques to provide insights into the factors driving membrane protein/lipid assembly and controlling the stability of the complexes.

抗生素, 在线固相萃取, 高效液相色谱法, 生物分析, 大体 积进样，建立更简便、灵敏的测定细胞裂解液样本中替加环素的 方法——在线固相萃取高效液相色谱法。

公司主要代理产品： 试剂盒：ELISA试剂盒、检测试剂盒、免疫组化试剂盒、放免试剂盒、分子生物学试剂盒、金标检测试剂盒、Omega试剂盒、细胞凋亡试剂盒、生化试剂盒。 各种动物血清：内皮细胞专用血清、HyClone、GIBCO胎牛血清。 抗体：进口原装抗体、进口分装抗体、单克隆抗体、多克隆抗体、单标抗体、双标抗 体、多标抗体、一抗、二抗；免疫组化抗体、免疫荧光抗体、流式细胞抗体、免疫细胞化学抗体。 细胞：原装进口细胞、肿瘤细胞、正常细胞、肿瘤耐药细胞 生化试剂：原装进口生化试剂、进口分装生化试剂。

肿瘤干细胞分选后进行新抗癌药物的研究是当今研究最前沿的领域。筛选获得的肿瘤干细胞数量极其少，对其进行信号通路蛋白翻译后修饰分析是全球该领域内的难点。本篇Nature protocol提供了肿瘤干细胞信号通路蛋白翻译后修饰完整解决方案。

生物界中绝大多数反应发生在由磷脂双分子层构成的细胞膜，或者细胞内部。细胞膜会影响到蛋白质的折叠，并且会创造出适合细胞反应生存的微环境。为了进一步了解和模拟真实的细胞膜结构，使用KSV NIMA LB膜分析仪学习细胞生物分子的相互作用。

利用高光谱成像仪获取伪装目标的高光谱数据，并进行目标识别。利用植被的红边效应可以区分开真实植被与伪装目标。伪装目标并未表现出非常明显的红边效应。

SpectraMax? MiniMax? 300细胞成像系统和SoftMax? Pro软件为EarlyTox细胞完整性试剂盒的应用提供了成像和数据分析的整套硬件软件工具。使用仪器的双通道荧光成像功能以及软件的单细胞分析功能，可在5分钟内分析一块96孔板细胞活性。 特点 信号强，缩短曝光时间，成像速度更快 适用于各种细胞类型，应用广泛 拍照和分析一体完成，流程简化

在发达国家，气体吸入式的麻醉非常普遍，与传统的药物注射麻醉方式相比，具有以下显著的共同优点： * 动物进入麻醉状态较快，苏醒也迅速，一旦停止麻醉，一般2分钟内动物即可苏醒 * 麻醉深度容易控制，若在手术过程中发现动物状态不佳，可马上停止麻醉或者快速充氧进行抢救，因此安全性非常好； * 动物的发病率和死亡率低，动物手术的成功率高； * 更重要的是，吸入式麻醉剂在体内不参与代谢，几乎完全由肺泡经呼吸排出，对实验结果不造成影响，研究成果易得到国际认可。

本文使用QCM-D实时分析了酶-蛋白在功能性生物表面的相互作用，特别的研究了功能性表面控制抗体的吸附和构象和抗体的酶降解作用。这些研究揭示了化脓性链球菌的防御机理，以及IDES酶如何切割免疫球蛋白。

抗体和不同种类的抗原的相互作用吸引了医疗方面和研究机构的广泛兴趣。使用筛查工具来检测他们的相互作用变得非常有意义。本文中使用了QCM-D结合自动进样器进行了稳定重复的抗体抗原相互作用反应。