绿茶中环糊精的HPLC-ELSD分析

表面活性剂是商用洗涤剂的主要成分，由于具有洗涤性能、吸湿性、渗透性、溶解性、分散性、润滑性、抗静电性、杀菌性和防锈性等多种性能，被广泛应用于各种工业和制药领域。非离子表面活性剂具有亲水基团，即使溶解在水中也不会离子化。由于它几乎不受水硬度和电解质的影响，因此可以与所有其他表面活性剂一起使用。鉴于其性质，非离子表面活性剂的使用量急剧增加，并指出了其对环境的影响。自2003年以来，非离子表面活性剂一直是《水质控制标准》的检测项目。这一次，使用具有二氧化硅NH2柱的ELSD检测器在HILIC模式下测量和分析作为非离子表面活性剂样品的Triton X-100和 Polyethylene Glycol 400。UASGOApplication Note 2/3Application NoteAnalysis of Cyclodextrines in Green Tea using HPLC-ELSD Analysis of Cyclodextrines in Green Tea using HPLC-ELSD Surfactants are well known as a main constituent of commercial detergent a nd is widely used in various in d ustrial and pharmaceutical f ield s due to various capabilities such as washing performance， moisture absorbency， osmosis， solubility， dispersibility，lubr i c i ty， antistatic ability ， bactericidal property and anti -rust property. Nonionic surfactants have hydrophilic groups which are not ionized even when dissolved in water . Since it is hardly aff e cted by water hardness and electrolytes， it can be used together with all other surfactants. Consider i ng its nature ， the usage of nonionic surfactants have i ncre a sed dram a tically and its affect on the environment has been pointed out. Since 2003，nonionic surfactants have been the test i tem for Water Quality Control Standards. This time ， Triton X-100 and Polyethylene Glycol 400 as no n ionic surfactant samples were measured and analyzed using an ELSD detector with Sil i ca NH2 column in HILIC mode. Keyword ： Cyclodextrine， HILIC， polymer NH2column， ELSD， a-Cyclodextrine， b-Cyclodextrine，g-Cyclodextrine PU-2089 Pump： PU-2089 Column： Shodex Asahipak NH2P-50 4E (4.6mmlD x250 mmL) Autosampler： AS-2057 Eluent： Water/Acetonitrile (40/60) Column oven： CO-2060 Column temp.： 50°C Detector： ELS-2040 ELSD condition： Nebulizer temp.： 30℃ Evaporator temp.： 30℃ Injection volume： Gas flow rate； 1.4 SLM 10pL Standard sample： a， b， g-Cyclodextrine 0.5 mg/mL each in Water/Acetonitrile (40/60) Resul t s and D i scussion Figu r e 1 s h ows t he chr o m at ogr a m o f a，b， g -C y cl o d e xtr i n e . F i g u re 1. C hro m a tog r am of th e standard mix t ur e o f Cycl o d e x t rine 1： a-Cyclod e xtrin e ， 2： g -Cyclod e xtrine ，3： b-Cyclodex t ri n e Figu r e 2 s h ows th e chr om a t og r a m o f gr e e n t e a m ix e d with c y c l o d ex tr i n e . A l l th e c o m p o n en ts wer e de t e c t ed wi th th e q ua n t i t a t i ve r es u l t ： a -Cy clod e x t r i ne 4.5 m g /10m L ， b -Cy c l o d e x tr ine 5.0 mg /10mL，g -C y cl o de x tr i n e 22.7 mg/10m L. Figure 2. C h romatogra m of green tea m i x ed wit h cyclodex tr i n e1： a-Cyclodext r i n e， 2： g-Cyclodex t ri n e ， 3： b-Cyc l odextri n e Sa m ple p rep arat i o n ： G r ee n te a combined with cyclod e xtr i ne was mixed with mobile ph ase of the same amount and was t h e n s e n t t h rou gh a 0.45pm f i lt e r.