对羟基苯甲酸酯的分析

防腐剂是一种常用的添加剂，用于提高食品的保质期。防腐剂用于防止微生物生长，微生物生长会导致食品腐烂和酸败。商业食品生产中使用的许多防腐剂都具有一定的毒性，它们的使用受到美国食品药品监督管理局等监管机构的严格控制。在本申请说明中，最常用的食品防腐剂之一对羟基苯甲酸酯的分析是根据下文报道的《健康科学分析方法》中描述的食品添加剂测试方法进行的。Application Note 2/3 Analysis of p-Hydroxybenzoate Ester Preservatives are a common addi ti ve used in improving food she l f-life. Preservat i ves are used to prevent microbial growth which can lead to putrefaction and rancidification of foodstuffs. Many of the preservatives used i n commercial food manufacture have a certain level of toxicity and their use is strictly controlled by regulatory agencies such as the FDA. In this application note， the analysis of one of the most commonly used food preservatives p-Hydroxybenzoate Ester was made according to the food add i t i ve test method described i n the Methods of Analysi s i n Health Science as reported below. Keywords Food additive， Preservative， p-Hydroxybenzoate Esters， C18column， UV detector， HPLC， isocratic separation Application Note Analysis of p-Hydroxybenzoate Ester Experimental Equipment Pump： PU-2080 Degasser： DG-2080-53 Column Oven： CO-2060 Autosampler： AS-2057 Detector： UV-2075 Column： CrestPak C18S (4.6 mml.D. x150 mmL， 5 pm) Eluent： Acetonitrile/5 mmol/LCitrate buffer (pH 4.1) (60/40) Flowrate： 1.0mL/min Column Temp.： 40℃ Wavelength： 270nm Injection Volume： 10 pL Standard Sample： p-Hydroxybenzoate ester1 ug/mL each in water(methyl， ethyl， propyl， butyl，isobutyl) Structural formulas of p-Hydroxybenzoate Ester are as follows (Fig. 1). Methyl p-hydroxybenzoate Ethyl p-hydroxybenzoate Propyl p-hydroxybenzoate Butyl p-hydroxybenzoate Isobutyl p-hydroxybenzoate Fig. 1 [3D mapping data analysis] Program Application Note Analysis of p-Hydroxybenzoate Ester Resu l ts The chromatogram of a s t andard mixture of p-Hydroxybenzoate Esters is shown i n Fig. 2. Five components were eluted with good resolution i n under 10 minu t es u s ing an isocratic m ethod. Fig. 2. Ch r omatogram of Standard Mixture of p-Hydroxybenzoate Ester