Benzoic Acid, Sorbic Acid and Dehydroacetic Acid的分析

在食品添加剂中，有几种食品防腐剂。虽然其中许多是有毒的，但它们具有抑制导致食物腐烂的各种微生物增殖的功能。因此，此类防腐剂的使用受到限制，目标食品受《食品卫生法》的监管。本报告根据《卫生化学分析标准方法》中所述的食品添加剂的检测方法，对苯甲酸、山梨酸和脱氢乙酸这三种食品防腐剂进行了分析。Application Note 2/2 Analysis of Benzoic Acid， Sorbic Acid and Dehydroacetic Acid In t rod u c t i o n Among the food addi t ives， there are severa l food preservatives. While many of them are toxic，they have a function to inhibi t proliferation of vario u s microorganisms which cause food to rot.Accordingly， t h e usage of su c h preservatives is limited and the t a rget foods are regulated by the Food Sani t ation Law. This report describes the analysis of three kinds of food preservat i ves ， Benzoic Acid， Sorbic Ac i d and Dehydroacetic Acid， according to the t e st i ng method for food additiv e s described in the Standa r d methods of Analysis for Hygienic Chemistry. Jas c o PU-2080 Plus pump Keywords： HPLC， Food additive， preservat i ve， Benzoic acid，Sorbic acid， Dehydroaceticacid，UVdetection， C18 column Application Note A n a l y s i s o f B e n z o i c Aci d ， S o rbi c Ac i d and D eh y droac e t i c Ac id E xperimenta l Eluent Pump： PU-2080 Degasser： DG-2080-53 Column Oven： CO-2060 Autosampler： AS-2057 Detector： UV-2075 Column： CrestPak C18S (4.6 mml.D. x 150 mmL， 5 pm) Eluent： 5 mmol/LCitrate buffer (pH 4.1) (70/20/10) Eluent Flow Rate： 1.0 mL/min Column Temperature： 40°℃ Wavelength： 230nm Injection Volume： 10 pL Benzoic acid， Sorbic acid， Dehydroacetic acid 2 ug/mL each in water Fig. 1 shows t h e s tr u c t ure of e a ch preser v a tive. COH CCH. H，C OH H，C ○ Be n zoic ac i d S o r bic ac i d Dehy d roacet i c acid R esu l ts The chrom a togram o f s t an da r d m ixt ur e di sp l aye d in Fig.2 s h o ws a goo d separ a t i on o f t h e th ree c om p onent s with in 9 m inu tes . JASCO INC. A p plicat i o n L i br a r y ： htt p ：//www.j as co i n c .c o m /ap p l i c a ti o n s