高效液相色谱-蒸发光散射检测法分析甘油三酯

尽管甘油三酯是一种能量来源，但人们认识到摄入过多可能会导致动脉硬化。由于甘油三酯中的大多数成分几乎没有紫外线吸收，因此示差折光检测器通常用于甘油三酯分析。然而，使用这种检测器需要很长时间才能稳定基线，并且异物经常会影响结果。ELSD是一种有效的检测方法，利用其高灵敏度和稳定的基线，解决了包括甘油三酯在内的脂肪分析问题。Application Note 2/3Application NoteAnalysis of Triglycerides by High Performance Liquid Chromatography Analysis of Triglycerides by High Performance Liquid Chromatography with Evaporative Light Scattering Detectio n In t rod u c t ion Altho u gh triglycerides function as an energy source， it is recognized that too much intake may cause arteriosclerosis. Since most of compo n ents in triglycerides have almos t no UV absorption， a differential refractive index detector is often used for triglycerides analysis. With this detector， however ， it takes a long t ime to stabilize the baseline and foreign substances often affect the results. ELSD is known as an effective detection method to solve the problems on fatty a n alysis including triglycerides， taking advantage of its high sensi t i vity and stable baseline. Keyword： Triglycerides， ELSD， Trilaurin， T r ilinorein，Tr i mylistin， T r iolein， T r ipalmit i n， T r istearin Jasco PU-2089 Experimental Equipment： Conditions： P um p ： PU-2089 Autosampler ： AS-2057 Co l umn oven ： AS-2057 D e tector： ELS -2040 Column： CrestPak C18S (4.6 mmlDx 150mmL， 5 um) Eluent： A； Acetonitrile， B； THF* Gradient condition： (A/B)， 0 min (75/25)， 40 min (67/33)，40.05 min (50/50) 45 min (50/50) 45.05 min (75/25) 1 cycle： 60min Detector： ELS-2040 Flow rate： 1.0 mL/min Column temp.： ELSD condition： 40°C Nebulizer temp.： 30°C Evaporator temp.： 50°C Gas flow rate： 1.6 SLM10uL Injection volume： Standard sample： Trilaurin， Trilinorein， Trimylistin，Triolein， Tripalmitin 1.0 mg/mL each Tristearin 0.5 mg/mL * T H F sol v ent does n o t i nclude any a d d i tives. Figure 1 shows the chromatogram of 6 well separated components of a triglycerides standard mixtu r e. F ig u re 1. Chroma t ogram of 6 components of a triglycerides sta n d a r d mi xt ure.1： T r i la u ri n ， 2： Tr i li no r ein ，3： Tri m yl i s t in ， 4： Triolein， 5： T ripal m i t i n ， 6： Tristear i n Figure 2 and f ig u re 3 show t he chromatograms of r ice bran oil and margarine， respect i ve l y. Fi g u re 2. C hr omatog r a m o f ri c e bran oi l .， Th e Peak n um ber s are t h e same a s in f i g u r e 1.P re t r eat m en t ： 1.0 g o f r i c e b ran o i l wal d i ssolved i n 10 m L o f aceton e an d was t h e n fil t ra t ed t h rough a 0.45 p m me m br a n e f ilter. F i g u re 3. C h r o mat o g r a m o f m argarin e .， T he peak nu m be r s a r e t h e s a m e a s i n fi g u re 1.P r e t r e a t m en t： 0.5 g o f m a rg arin e was dis s o l v e d i n 10 mL o f a c e t one an d w a s t hen f il t r a t e d t h rough a 0.45 u m me m bran e f il t e r .