HPLC-ELSD法分析抗胀气化合物

药物含有各种活性成分，并且为了同时分析这些成分，需要高灵敏度检测的梯度洗脱方法。当以梯度模式分析药物时，蒸发光散射检测器（ELSD）是有用的，因为它可以以高灵敏度检测除挥发性成分外的几乎任何化合物，并提供稳定的基线。在本报告中，使用ELSD和PDA检测来分析抗胀气中所含的一些成分。硬脂酸被用作片剂的润滑剂，ursodeoxycholic acid 对改善消化和吸收有效，阿片醇被用作消毒剂，黄连素具有抗菌、抗炎和保护胃粘膜的作用。Application Note 2/3Application NoteAnalysis of Anti-flatuents using HPLC-ELSD Analysis of Anti-flatuents using HPLC-ELSD Medicines contain various active i ngredients and for analyz i ng such ingredients simultaneously， a gradient elution method requiring high sensitive detection is required. When analyz i ng medicines in gradient mode an evaporative light scattering d e tector (ELSD) is useful because it can d etect almost any compound with the exception volatile components with high sensitivity and provides a stable baseline. In this report， some ingredients contained in an anti -flatulent were analyzed using ELSD and PDA d e tection. Ste a ric acid is used as a lubricant for forming tablets， ursodeoxycholic acid is effective for improving digestion and absorption， acrinol is used as a disinfectant and berberine has antibacterial.ant i -inflammatory and gastric mucosa l protect i on effects. Keyword ： Antiflatuent ， C18 col u mn， PDA det e ctor，ELSD， berberine， acrinol ， ursodeoxycholic acid，Stearic acid PU-2089 Experimental Equipment： Conditions： P u m p ： PU-2089 Autosampler： AS-2057 Column oven： CO-2060 Detector： E L S-2040 Column： CrestPak C18S (4.6 mmlDx150 mmL， 5 pm) Eluent A： 10 mM Ammonium acetate in Acetonitrile Eluent B： 10 mM Ammonium acetate Gradient condition： (A/B)， 0 min (5/95) ，15 min (70/30)，15.05 min (95/5) 20 min (95/5)，20.05 min (5/95) 1 cycle： 35 min Flow rate： 1.0 mL/min Column temp.： 40°℃ ELSD condition： Nebulizer temp.： 30℃ Evaporator temp.； 60℃ Gas flow rate； 1.0 SLM PDA wavelength： 200-650nm Injection volume： 10 pL Standard sample： Stearic acid， Acrinol， Ursodeoxycholic acid， Berberine Figu r e 1 s h ows s tr u c tu ra l f o r m ul a o f t h e in gr e d i e n t s c o n ta i n e d i n t h e a n t i-f latu en t. Fig ure 1. S t ru c tural formula o f t h e i n gr edients c o nta i ned i n an t i -f latue n t. Re sults Figu r e 2 s h ows c h r o ma to g r a m o f t h e sta n da r d m i xt ure w h ile f i g ure 3 sh o w s c h r o m a t o gr a m o f a n t i-f l at u e n t . Th e t op c h r oma to gr a m i s f r om th e EL S D a n d th e bott om ch r oma t o g ram is f r o m th e PD A (a t 220n m ). O n ly two i n g red i e n t s we r e de t e c t e d by t h e P D A， w h i l e a l l fou r i n g r e d ie n t s w e r e c l ea r l y d e t e c ted b y t h e EL SD . Figure 2. Ch romatog r am o f t h e standard mixt u re. 1： S tear i c a c id [0.5 mg/m L]，2： A crinol [0.1 m g/mL]， 3： B erberine [0.1 m g/m L]， 4：Ursodeoxychol i c acid [0.1 mg/mL] F igure 3. C h romatogram of t h e anti -flatuent.1： Stear i c acid， 2： Ac r inol ， 3： B e rbe r i n e， 4：U rs o d e oxy chol ic acidPr e p a ration ： A 1.0 m g/mL so l u t ion was p rep a red by di ssolv i ng t he powder f r om t he crushed ant i -f l a t uen t i nto m ethanol and sent t h r oug h t he 0.45 p m membrane f i l ter.