弹性蛋白聚合物中凝聚层分析的新型高通量检测方案(图像粒度粒形)

DKSH New High-Throughput Method for Elastin-likePolymer (ELP) Coacervate Analysis 弹性蛋白聚合物(ELP)凝聚层分析的新型高通量方法 Posted by Michelle Devoe Michelle Devoe 发表 December 2018- A recent study by researchers from the University of NewEngland and University of New Hampshire has demonstrated that flow imagingmicroscopy isSaan accurate，Imore efficient，，and more informative method ofelastin-like polymer (ELP). coacervate analysis than standard methods. ELPcoacervates are a class of molecules with promising applicationsin drugdelivery vehicles， tissue engineering，environmentalremediation，i，and more.ELP coacervate architecture is stimuli-responsive and highly tunable， nmakingthem ideal for the above-mentioned applications 2018年12月-新英格兰大学和新罕布什尔大学的研究人员最近的一项研究表明，与标准方法相比，流动成像显微镜是一种准确，更有效，信息更丰富的弹性蛋白样聚合物(ELP)凝聚分析方法。 ELP 凝聚层在作为药物递送的载体，组织工程，环境修复等方面具有广阔的应用前景。 ELP 凝聚层结构具有刺激响应性和高度可调性，使其成为上述应用的理想选择。 ELP coacervates imaged by the FlowCam. Size and circularity， two of the 40+ properties that can be measuredby the FlowCam，is used to sort coacervates. Image from Marvin et al.(2018). ELP 凝聚了由 FlowCam 成像的图像。尺寸和圆度是FlowCam 可以测量的40多个属性中的两个，用于对凝聚层进行分类。图片来自 Marvin 等。(2018)。 Standard methods for ELP coacervate analysis areindirect and cumbersome.Data from Visible-UV spectrophotometer turbidity measurements and dynamiclight scattering(DLS)size measurements are superimposed to evaluate theformation of micron-scale aggregates， and particle geometry is constructedindirectly from diffusion data..IImaging analysis methods such as optical，electron，，and scanning probe microscopy are useful methodsto acquire sizeand shape data， however it istime-consumingand laborious to acquire alarge enough sample size to get statistically significant data. ELP 凝聚层分析的标准方法是间接和麻烦的。来自可见-紫外分光光度计浊度测量和动态光散射 (DLS)尺寸测量的数据被叠加以评估微米级聚集体的形成，并且颗粒几何形状间接地由扩散数据构建。 诸如光学，电子和扫描探针显微镜之类的成像分析方法是获取尺寸和形状数据的有用方法，然而获得足够大的样本大小以获得统计上有效的数据是耗时且费力的。 In this study by the University of New England and University of NewHampshire，the size，， morphology，and behavior of ELP coacervates subjectedto various solvent conditions were measured and observed using the FlowCamparticle analyzer..RResultss vwerevalidated by comparison with DLS and atomicforce microscopy analyses.Flow imaging microscopy was demonstrated to be asuccessful method for ELP coacervate analysis. Additionally， flow imagingmicroscopy reported additionaliffindings；tthat were not measured using theDLS， microscopy，l， Cor Visible-UV spectrophotometry. 在新英格兰大学和新罕布什尔大学的这项研究中，使用FlowCam 颗粒分析仪测量和观察经受各种溶剂条件的 ELP 凝聚层的尺寸，形态和行为。 通过与 DLS 和原子力显微镜分析的比较验证结果。 流动成像显微镜被证明是 ELP 凝聚层分析的成功方法。 此外，流动成像显微镜提供了 DLSs，，显微镜或可见紫外分光光度法不能获得的结果。 选择大昌华嘉，就是选择仪器应用专家Think Asia. Think DKSH.www.dksh-instrument.cn 电话： 邮箱： ins.cn@dksh.com 2018年12月 - 新英格兰大学和新罕布什尔大学的研究人员最近的一项研究表明，与标准方法相比，流动成像显微镜是一种准确，更有效，信息更丰富的弹性蛋白样聚合物（ELP）凝聚分析方法。 ELP凝聚层在作为药物递送的载体，组织工程，环境修复等方面具有广阔的应用前景。 ELP凝聚层结构具有刺激响应性和高度可调性，使其成为上述应用的理想选择。