聚苯乙烯低聚物的回收制备高效液相色谱

循环制备型高效液相色谱是一种分离技术，通过将洗脱液重复循环到同一柱中，可以实现多种成分的完全分离。这与使用较长或多个列具有相同的效果。当应用于制备型HPLC时，每种纯化的组分都可以非常有效地分馏。该技术具有以下特点：（1） 降低了列成本，因为不需要使用更长或多个列。（2） 减少了环境负荷和成本，因为在循环模式下，重复使用相同的流动相溶剂，同时改进了分离。（3） 有效的制备纯化，因为每种成分只有在改进分离后才能分馏。2/3Recycling Preparative HPLC of Polystyrene Oligomer Ap plication Note Recycling Preparative HPLC of Polystyrene Oligomer Intr odu cti o n Recycling Preparative HPLC is a separation technique that can achieve complete separation ofa number of components by repeatedly cycling the eluent to the same co l umn. This has the same effect as using longer or multiple columns.When applied to preparat i ve HPLC each purified component can be f ractionated very effective l y. This techniq u e has the following features： Jasco P U-2086 (1) Reduced col u mn costs， as it is not necessary to use longer or mul ti ple columns. (2) Reduces environmental loads and costs ，because in recycle mode ， th e same mobile phase solv e nt is used repeatedly while i mproving separation. (3) Effect i ve preparat i ve pur i f i cation because each component i s fractionated only after i mproving the separation. Application Note Experimenta l Instrument Conditions Eluent Pump：PU-2086Column：Megapak GEL 201C Autosampler：AS-2058(20 mmlD x500mmL)Detector：UV-2070Eluent：Chloroform (withsemi-prep.cell)Flow R ate：3.0mL/min Fraction Collector：ADVANTEC CHF 122SC Column Temperature：40°C Fraction Controller：FC-2088-30Wavelength：254nm Fraction Valve Unit ：HV-2088-06Injection Volume：200 uL Recycle Kit Gradient Condit i on：Polystyrene Oligomer (1.0%( w/v) in Chroloform) Figure 1. Flow d iag r am of t he R ec ycl in g P rep a r a t i v e HPLC sy ste m Exper i menta l Fig. 2 Recycle chromatogram of a Polystyrene Oligomer ， showing the i mprovement of sepa r ation by recycling six times i n approximately 180 minut e s. Three components te t ramer， pentamer and hexamer of Polystyrene Oligomer were c l early separated and fractionated. Figure 2. Rec y cle C hr oma t ogra m of Po l y sty r ene Oli g omer Recycling Preparat i ve HPLC is a separation technique that can achieve complete separation ofa number of components by repeatedly cycling the eluent to the same column. This has the same effect as using longer or multiple co l umns. When applied to preparat i ve HPLC each purified component can be fractionated very effectively. This technique has the following features： (1) Reduced column costs， as it is not necessary to use longer or multiple columns. (2) Reduces environmental loads and costs， because i n recycle mode， the same mobile phase solvent is used repeatedly wh i le improving separation. (3) Effective preparat i ve purification because ea c h component is fractionated only after improving the separation. A p plicat i o n L i br a r y ： htt p ：//www.j as co i n c .c o m /ap p l i c a ti o n s