青贮饲料和干草饲料中有机酸检测方案(毛细管电泳仪)

Analytical Equipment山<一www.lumexinstruments.com DETERMINATION OF ORGANIC ACIDS IN FEEDSTUFF， SILAGE， HAYLAGE，AND FERMENTATES FOR BIOGAS PLANTS INTRODUCTION To provide the proper conditions for silage or haylage storage in agriculture it is necessary to regularlymonitor the formation of certain organic acids in it. For example， the formation of butyric acid can indicatespoiling of material. Formic or propionic acids can be artificially added to increase the nutritional quality.The same materials and some other biodegradable waists (agricultural residues， sewage sludge， etc.) arealso used in biogas plants， where the fermentation process must be carefully monitored， in particular， bythe determining the certain organic acids. The present method allows determination of some important organic acids like acetic， lactic， butyric，propionic， and formic in feedstuff additives， silage， haylage and in different biodegradable materials ofbiogas plants. Some other organic acids (oxalic， fumaric， etc.) can be also determined within thismethod. MEASUREMENT METHOD The capillary electrophoresis method for the determination of organic acids is based on their differentialmigration and separation in a fused silica capillary under the influence of electric field. Samplepretreatment includes either just water extraction or sample distillation with steam. CE separation iscompleted within 5 minutes. Identification and quantification of the analyzed organic acids is performed by measuring their intrinsic UVabsorption at 190 nm. EQUIPMENT AND REAGENTS The CAPEL-105M capillary electrophoresis system with high-voltage negative polarity is used inmeasurements. Data acquisition， collection， processing and output are performed using a personalcomputer running under WINDOWSXP/7 operating system with installed dedicated software packageELFORUN All reagents must be of analytical grade or better. EXAMPLE OF A REAL ANALYSIS Buffer： special with CTAB The contents of this paper are subject to change without notice.The information in this leaflet is supplemental. To get more specific information on this method， please contact the developer of thismethod Lumex Instruments Group. NORTH AMERICA EUROPE RUSSIA CHINA Lumex Instruments Canada Lumex Analytics GmbH Lumex-Marketing LLC Beijing Lumex Analytical 0890278 B.C. Ltd. E-mail： info@lumexanalytics.de E-mail： sales@lumex.ru Equipment Co. Ltd. E-mail： eaa@lumexinstruments.com www.lumexanalytics.de www.lumexinstruments.com E-mail： lumex@lumex.com.cn www.lumexinstruments.com www.lumexcn.com INTRODUCTIONTo provide the proper conditions for silage or haylage storage in agriculture it is necessary to regularly monitor the formation of certain organic acids in it. For example, the formation of butyric acid can indicate spoiling of material. Formic or propionic acids can be artificially added to increase the nutritional quality.The same materials and some other biodegradable waists (agricultural residues, sewage sludge, etc.) are also used in biogas plants, where the fermentation process must be carefully monitored, in particular, by the determining the certain organic acids.The present method allows determination of some important organic acids like acetic, lactic, butyric, propionic, and formic in feedstuff additives, silage, haylage and in different biodegradable materials of biogas plants. Some other organic acids (oxalic, fumaric, etc.) can be also determined within this            method. MEASUREMENT METHODThe capillary electrophoresis method for the determination of organic acids is based on their differential migration and separation in a fused silica capillary under the influence of electric  field.  Sample  pretreatment includes either just water extraction or sample distillation with steam. CE separation is    completed within 5 minutes.Identification and quantification of the analyzed organic acids is performed by measuring their intrinsic UV absorption at 190 nm.