饲料及饲料添加剂中有机酸检测方案(毛细管电泳仪)

重Analytical EquipmentHIGH PERFORMANCECAPILLARY ELECTROPHORESISSYSTEMwww.lumexinstruments.ccm DETERMINATION OF ORGANIC ACIDSIN FEEDSTUFFS AND FODDER ADDITIVES Lumex Method M 04-74-2012 INTRODUCTION The method is used for the determination of the mass concentration of oxalic， formic， fumaric， succinic，malic， citric， acetic， propionic， lactic， benzoic， sorbic， butyric acids in fodder additives and butyric acid insilage and haylage by capillary electrophoresis. For the determination of ascorbic acid and its salts in food additives and fodder additives use the methodM 04-86-2016 (Lumex Instruments set， order No 0300002717). MEASUREMENT METHOD The measurement method is based on capillary zone electrophoresis with direct UV detection at thewavelength of 190 nm. MEASUREMENT RANGE The measurement ranges for the components are presented in the table below. Compound Measurement range，% Compound Measurement range， % Acetic acid 0.1-80 Lactic acid 0.12-80 Benzoic acid 0.005-50 Malic acid 0.05-80 Butyric acid 0.05-50 Oxalic acid 0.03-10 Citric acid 0.05-80 Propionic acid 0.1-80 Formic acid 0.15-80 Sorbic acid 0.025-50 Fumaric acid 0.005-80 Succinic acid 0.05-80 Inorganic anions (chloride， sulfate， nitrate and phosphate) in concentrations， typical for the mentionedsamples do not deteriorate organic acids determination. EQUIPMENT AND REAGENTS The CAPEL capillary electrophoresis system is used in measurements. Data acquisition， collection，processing and output are performed using a personal computer running under WINDOWS XP/7/8/10operating system with installed dedicated software package ELFORUN. Lumex Instruments set， order No 0300001897. EXAMPLES OF REAL ANALYSES BGE： phosphate， with CTAB Capillary： -eff/ -tot 40/50 cm， ID 50 um Injection： 150 mbar x sec The contents on this paper are subject to change without notice.To get more specific information， please contact the representative by sales@lumexinstruments.com ' National Standard GOST R 56373-2015. DETERMINATION OF ORGANIC ACIDSIN FEEDSTUFFS AND FODDER ADDITIVESLumex Method M 04-74-2012INTRODUCTIONThe method1 is used for the determination of the mass concentration of oxalic, formic, fumaric, succinic,  malic, citric, acetic, propionic, lactic, benzoic, sorbic, butyric acids in fodder additives and butyric acid in  silage and haylage by capillary electrophoresis.For the determination of ascorbic acid and its salts in food additives and fodder additives use the methodМ 04-86-2016 (Lumex Instruments set, order No 0300002717).MEASUREMENT METHODThe measurement method is based on capillary zone electrophoresis with direct UV detection at the wavelength of 190 nm.MEASUREMENT RANGEThe measurement ranges for the components are presented in the table below. CompoundMeasurement range, %CompoundMeasurement range, %Acetic acid0.1–80Lactic acid0.12–80Benzoic acid0.005–50Malic acid0.05–80Butyric acid0.05–50Oxalic acid0.03–10Citric acid0.05–80Propionic acid0.1–80Formic acid0.15–80Sorbic acid0.025–50Fumaric acid0.005–80Succinic acid0.05–80Inorganic anions (chloride, sulfate, nitrate and phosphate) in concentrations, typical for the mentioned samples do not deteriorate organic acids determination.EQUIPMENT AND REAGENTSThe CAPEL capillary electrophoresis system is used in measurements. Data  acquisition,  collection,  processing and output are performed using a personal computer running under WINDOWS® XP/7/8/10 operating system with installed dedicated software package ELFORUN.Lumex Instruments set, order No 0300001897.EXAMPLES OF REAL ANALYSESBGE: phosphate, with CTAB Capillary: Leff/ Ltot 40/50 cm, ID 50 µm Injection: 150 mbar x secVoltage: – 17 kV Temperature: + 20℃Detection:         190 nm