姜黄提取物中主要成分分析检测方案(液相色谱柱)

姜黄提取物 Turmerlc extract HPLC Conditions ba065 Column ： CAPCELL PAK C MGII S5： 4.6 mm i.d.x 150 mm Mobile phase ： Trifluoroacetic acid was added to a solution (H：0 / CH：CN= 55 /45) at 0.1 vol% Flw rate ： 1.0 mL/min Temperature ：40℃ Detection ： UV 420 nm Inj. vol. ：5pl Pretreatment ： The standard compound was dissolved in the mobile phase at 20pg/mL. The content of the supplement was diluted with methanolso that the concentration of curcumin be 1 mg/mL， approximately，and sonicated for five minutes. After leaving it for a while theupper layer was filtered with a 0.2-um filter， diluted with methanolat 20 pg/mL， and introduced to HPLC ※1 pg/mL=1 ppm