方案详情
文
The Biochrom 30 Amino Acid Analyser is a well
established analytical instrument for clinical
diagnosis as its IVD approval allows the diagnosis
of inborn errors of metabolism such as
Phenylketonuria.
This robust and reliable instrument is also capable
of other types of applications far from the clinical
market e.g. its usage in the food industry allows
routine analysis of amino acid content in food and
feedstuff in quality control laboratories.
The Biochrom 30 can also be used for analysing
rare, unusual amino acids or derived molecules for
research purposes.
In order to develop new methods for analysing
molecules of interest in the scientific market, a
new application has been developed to analyse
the theanine content in green tea leaves.
Theanine is a molecule derived from the amino
acid glutamine and is usually found in tea
(infusions of Camellia sinensis), and also in the
basidiomycete mushroom Boletus badius.
Because it can enter the brain, theanine has
psychoactive properties. Theanine has been
shown to reduce mental and physical stress, may
produce feelings of relaxation, and improves
cognition and mood when taken in combination
with caffeine. These effects are believed to be
caused by the ability of theanine to increase GABA
production in the brain.
The chemical structure of theanine allows it to be
separated and detected in green tea samples
using a Biochrom 30 Amino Acid Analyser.
方案详情
Market Area： Food industryApplication Note： B30.19 Analysis of Theanine inGreen Tea using theBiochrom 30 Amino AcidAnalyser. biochrom Jean-Philippe Veyssier， Biochrom Ltd. Cambridge， UK Introduction The Biochrom 30 Amino Acid Analyser is a wellestablished aanalytical instrument for clinicaldiagnosis as its IVD approval allows the diagnosisofinborn| errors of metabolism such asPhenylketonuria. This robust and reliable instrument is also capableof other types of applications far from the clinicalmarket e.g. its usage in the food industry allowsroutine analysis of amino acid content in food andfeedstuff in quality control laboratories. The Biochrom 30 can also be used for analysingrare， unusual amino acids or derived molecules forresearch purposes. In order to develop new methods for analysingmolecules of interest in the scientific market， anew application has been developed to analysethe theanine content in green tea leaves. Theanine is a molecule derived from the aminoacid glutamine andisisuusually found inntea(infusions of Camellia sinensis)， and also in thebasidiomycete mushroom Boletus badius.Because it can enter the brain， theanine haspsychoactivepproperties.. Theaninehasbeenshown to reduce mental and physical stress， mayproduce feelings of relaxation，and improvescognition and mood when taken in combinationwith caffeine. These effects are believed to becaused by the ability of theanine to increase GABAproduction in the brain. The chemical structure of theanine allows it to beseparated and detected in green tea samplesusing a Biochrom 30 Amino Acid Analyser. Experimental A Biochrom 30 Lithium High Performance systemwas used for the analysis. It is equipped with a 20cmx4.6mm I.D LiHPcolumn and a set of Lithium Citratebuffers. The standard High Performance elutionprogram was used. After the extraction of the free amino acid contentfrom finely ground tea leaves in Lithium citrate buffer，the extracts were deproteinised with sulfosalicylicacid solution containing Norleucine as the internalstandard. The samples were filtered through a0.22um membrane. 40pL was injected onto thecolumn. 650 Minutes Results Theanine elutes in 41min and is clearly separated from any other amino acids presents in the tea. It alsogives a high response when reacting with the Ninhydrin reagent. Furthermore， the method allows theidentification of 20 other amino acids naturally present in tea in a single run. The extraction method is simple and with the specific ion exchange chromatography method coupledwith Ninhydrin detection， no matrix interference is detected with other types of molecules. Once again， the Biochrom 30 reveals its flexibility， allowing the separation scientist to use it for varioustypes of applications， without the inconvenience of heavy method development work. If you require more information about this application， please contact us atsupport@biochrom.co.uk www.biochrom.co.uk Biochrom Ltd， Cambridge Science Park， Milton Road， Cambridge， CB FJ UK Tel +()Fax+()
确定
还剩1页未读，是否继续阅读？
产品配置单
大昌华嘉科学仪器为您提供《绿茶中氨基酸检测方案 》，该方案主要用于茶叶中营养成分检测，参考标准--，《绿茶中氨基酸检测方案 》用到的仪器有全自动氨基酸分析仪 Biochrom 30+
推荐专场
相关方案
更多
该厂商其他方案
更多