其他食品中过敏原检测

香菇中的脂肪所含脂肪酸，对人体降低血脂有益。其脂肪含量测定是经过酸水解，冷却、过滤、洗涤残渣，再经石油醚提取，烘干、恒重得到脂肪含量。

阿斯巴甜、三氯蔗糖、安赛蜜等人工甜味剂属于日本食品卫生法中的指定添加物，相关部门针对允许使用甜味剂的部分食品及使用量制定了使用标准。对于在日本以外的部分地区使用的甜蜜素等其他人工甜味剂，在日本国内属非指定添加物，需对特定的进口食品进行检测。因此，不仅对日本允许使用的人工甜味剂，还是非指定添加物，均需进行定量检测。本文向您介绍使用岛津 LCMS-8050对16种甜味剂进行同时分析的示例。

本文使用配备了示差折光、四毛细管桥粘度及双角度激光光散射三个检测器的 Agilent 1260 Infinity 多检测器 GPC/SEC 系统，配合 PL Aquagel-OH 系列色谱柱，分别以两种不同盐浓度的缓冲液作为流动相，对羧甲基淀粉钠的分子量、分子量分布和分子构造进行了表征，并研究了流动相盐浓度对分子构象的影响。通过与仅配单一示差检测器的GPC/SEC 系统对比得到，1260 多检测器 GPC/SEC 系统，无需建立标准曲线，即可得到更加准确的样品分子量及分子量分布检测结果，同时还可得到样品分子构造和分子构象信息

There are many advantages in miniaturizing sample preparation. The use of less solvent reduces solvent cost and waste. Smaller sample sizes are easier to handle, store, and process in the lab. Reduction in sample amount provides cost savings on sample preparation sorbent and substantial decreases in cost associated with the use of labeled compounds as internal standards. With a smaller sample size, it may be possible to use additional labeled compounds for troublesome analytes that are cost prohibitive with larger sample sizes. We analyzed pesticides in food using miniaturized QuEChERS extraction and thultra-efficient ionization source of the Agilent 7010 Triple Quadrupole GC/MS, which reduces injected sample volume by 75%. On average, limits of quantitation (LOQ) ~10 ng/g were reached for 95% of the 126 pesticides studied in apple, carrot, and broccoli. Less matrix injected delivers prolonged uptime and sustained performance and, therefore, lower maintenance costs. By injecting only 25% of the standard 2 μL injection volume and implementing our recommended pesticide analysis method, we analyzed the pesticides at or below threshold MRLs of the EPA, EU, and Japan, 0.01 mg/kg (10 ng/g), which was adequate to monitor exposure.

This Application Note describes an UHPLC/Q-TOF/MS method for screening 240 pesticides and pesticide metabolites in difficult food matrixes. The method benefits from increased chromatographic resolution using the Agilent 1290 Infinity UHPLC System and improved ionization capabilities with an Agilent Jet Stream ionization source. More importantly, the successful screening of analytes is accomplished by the innate sensitivity improvement of the Agilent 6545 Q-TOF LC/MS System and ion transmission tuning to facilitate the optimal ion transmission of small, fragile organic molecules. Targeted MS/MS acquisition was used for analyte quantitation and confirmation. Black tea matrix was chosen for its complexity, and avocado matrix was chosen to represent food commodities with high lipid content. Our results demonstrate that the improved detection of small organic molecules by the 6545 Q-TOF LC/MS System allows the screening and quantitation of most of the targeted pesticides below the maximum residue limits (MRLs) specified by the European Commission regulations.

The presence of hidden nut and tree nut allergens in foods can result in serious health issues, necessitating a method capable of detecting and quantifying them at trace levels using a single robust assay. To find specific peptide markers that can be used to determine the presence or absence of specific nuts in food, proteins unique to 11 tree nuts (almond, pecan, cashew, walnut, hazelnut, pine nut, brazil nut, macadamia nut, pine nut, chestnut, and coconut) and peanut were enzymatically digested and analyzed using accurate - mass Q - TOF LC/MS. Each marker peptide was selected by establish ing its presence in raw and roasted nuts, processed and unprocessed food, abundance (sensitivity), sequence size, and uniqueness to a specific nut. The National Center for Biotechnology Information (NCBI) nr database searches were performed to confirm pep tide identities, and to ensure that the marker peptides chosen were not present in other nuts or common food ingredients such as barley, corn, rice, soy, and wheat. Two marker peptides were selected for each tree nut, and four were selected for peanuts. An alysis of peptide digests from grains such as barley, corn, rice, quinoa, soy, and wheat did not present interfer - ences. The peptide markers were tested to determine if they could be used to screen common foods for the presence of the 11 tree nuts and pea nut at sub - ppm levels. Foods containing nuts as listed on the label showed a response for the cor - rect nut. Foods processed using equipment also used to process other tree nuts or peanuts were in certain cases found to contain these other nuts.

This application note describes a method for the simultaneous determination of 26 carbamates and their metabolite residues in ginger by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) based on the work published by He, et al . [1]. The ginger samples were initially extracted using acetonitrile, followed by cleanup using NH2 solid phase extraction (SPE) column. The samples were then enriched by nitrogen evaporation, redissolved in a mixture of acetonitrile:water (1:1, v:v), and analyzed by UHPLC-ESI-MS/MS. With matrix-matched standard calibration for quantitation, the method showed excellent linearity, with correlation coefficients of》=0.99 for all compounds in the examined concentration range. The limits of detection and limits of quantitation were in the ranges of 0.050–2.0 μg/kg and 0.20–5.0 μg/kg, respectively. At spiking levels of 10, 30, and 100 μg/kg, the recoveries for all compounds ranged from 70.9–119% with RSD of 1.0–11%. The method is simple, rapid, and sensitive, thus it can meet the requirements for the determination of carbamates and their metabolite residues in ginger.

The Agilent 1290 Infi nity II LC was used to analyze additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste. The developed method facilitates accurate and sensitive determination of nine additives using a diode array detector (DAD) and an evaporative light scattering detector (ELSD) placed in series. After the DAD, a valve was installed to remove nonretained solutes in reversed-phase LC, which could increase the noise level of the ELSD. Total analysis time, including re-equilibration, was below 10 minutes with minimal sample preparation. Method performance was evaluated with standard solutions as well as with a series of real samples.

一些金属对人体来说不可或缺，而另一些则含有剧毒。因此，在食品生产的每一个阶段都需要监测痕量金属元素。 借助安捷伦的原子光谱产品系列产品 – AA、MP-AES、ICP-OES、ICP-MS（包括 ICP-MS/MS）和 HPLC-ICP-MS，能够快速筛查食品和饮料。 1，快速筛查有毒和有益的金属元素 2，分析大量元素和食品类型 3，测定痕量、微量及常量元素的浓度

岛津高效液相色谱-三重四极杆质谱联用仪LCMS-8040 同时分析九种属于指定添加物及非指定添加物的人工合成甜味剂。

Nexera-e使用全二维液相色谱法，具有一维和二维两种不同的分离模式，通过组合多维分离系统，可对常规一维LC难以分离的组分进行分离。 蒸发光散射检测（ELSD）方法是将色谱柱中溶出的洗脱液进行蒸发，从而对目标化合物进行微粒化，这种测定散射光的方法对无紫外吸收的甘油三酯检测很有效。

氨基酸是食品中的重要的营养物质，氨基酸的种类和数量是衡量食品营养价值高低的重要指标，目前，关于氨基酸的检测方法有氨基酸分析仪测定，高效液相色谱法等，其中高效液相色谱法分离度高，选择性好，并分为柱前衍生方法和柱后衍生方法。常用的柱前衍生方法有邻苯二甲醛( OPA) 、丹酰氯、9-芴甲基氯甲酸酯、2,4-二硝基氟苯等。

矿泉水瓶等容器产品的瓶盖开启扭矩值高低是相关生产企业产品管控的重点指标之一。开启扭矩值是否合适将直接影响到生产线及销售环节产品质量，开启扭矩过高则不便于使用者开启，开启扭矩过低则产品瓶口不密封。

该方法可用于测定液体样本（各种水质、饮料和生物液体）的重金属，检测矿化后的其他样本，如土壤、沉积物、废水、食物、原料、生物组织和石油化学类。采用采用塞曼高频偏振光谱技术（ZHFMPS）和电热原子化技术可对某些复杂样本直接分析，抗干扰性强。采用先进的ZAAS-HFM分析技术，使检测复杂样品更加简单，与其它的分析技术相比，MGA-915MD可用适当的成本和工作量，满足对低含量样品（ppb级）的检测需求。采用的激发光源--高频无极放电灯（EDL），其发射的高强度激发谱线，无需氢化物发生可直接检测砷、硒、锑等元素，消除了因氢化物发生所带来的试剂、温度等干扰因素，使结果更加准确。

MA2蛋白高度测定仪操作简单，价格便宜，测量速度快 1）蛋白高度测定仪1台 尺寸：180mm 重量：400g 2）蛋质测定台：尺寸：330×300×20mm 3)哈市单位计算 1个

本实验中的甜菊糖苷是以甜叶菊干叶为原料，经提取、精制而得的食品添加剂，主要糖苷为甜菊苷和瑞鲍迪苷A。甜菊糖苷的组分测定对甜菊品种改良和甜菊糖苷的生产与应用具有重要意义。本文参照GB8270-2014标准，使用东西分析LC-5510型高效液相色谱仪建立测定食品添加剂甜菊糖苷中甜菊苷含量的方法。

与传统的干法、湿法消解相比，高压消解具有操作简单、消解完全、易挥发元素损失少、污染少和节能快速等优点。本实验参考国标GB5009.12《食品安全国家标准食品中铅的测定》进行检测，同时进行加标回收率实验Pb回收率为100.9%。

以ＧＢ／Ｔ　５００９．１２－２００３食品中铅的测定和ＧＢ　２７６２－２００５食品中污染物限量的国家标准为实验依据，使用国内外多家仪器厂家的石墨炉原子吸收光谱仪对食品中铅含量进行了测定。比对研究证明，在食品领域，国内石墨炉原子吸收光谱仪的测试精密度和准确度能够满足国家标准对食品中铅限量测试的要求

柱温：190 oC ( 5 min ) - 250 oC, 8oC/min ( 5 min ) 载气：H2, 42cm/sec, 40 oC 进样方式：分流, 50:1, 260 oC 样品：糖醇乙酸酯衍生物, 0.5 μL 检测：FID, 16 x 10-11 AFS, 260 oC

柱温：50 oC - 92oC, 3oC/min - 220 oC, 20 oC/min ( 1 min ) 载气：He, 36cm/sec 进样方式：吹扫捕集进样 解吸 温度：220 oC 检测：MSD, 280 oC 离子：源EI, 70eV 扫描范围：35 - 260 AMU

柱温：70 oC ( 15 min ) - 190 oC, 2oC/min ( 5 min ) 载气：He, 20 cm/sec, 70 oC 进样方式：分流, 20:1, 220 oC 样品：挥发性风味物质混合物, 0.8μL 检测：FID, 64 X 10-11 AFS, 260 oC

柱温：165 oC ( 30 min ) - 220 oC,1.5 oC/min ( 15 min ) 载气：He, 40 cm/sec 进样温度：225 oC 检测：230 oC

柱温：165 oC ( 30 min ) - 220 oC,1.5 oC/min ( 15 min ) 载气：He, 40 cm/sec 进样温度：225 oC 检测：230 oC

柱温：120 oC, ( 3 min ) - 220 oC, 20 oC/min, ( 12 min ) 载气：He, 34cm/sec, 1mL/min 进样方式：分流, 100:1, 250 oC 样品：沙巴棕, 1.0 μL 检测：300 oC

柱温：145 oC, 恒温 载气：H2, 40 cm/sec 进样方式：分流, 50:1, 250 oC 样品：游离脂肪酸, 1.0 μL, 10-20ng/μL 检测：FID, 2 x 10-11 AFS, 250 oC

流动相：甲醇+0.1%甲酸水溶液=10:90 流速：1.0 mL/min 温度：室温 检测器：UV 254 nm

乳化喷雾干燥法制备微胶囊最适于亲油性液态物料的微胶囊化，芯材的憎水性越强，包埋效果越好。喷雾干燥过程很短，只有数秒时间，生产可连续进行，产品可免于长时间受热，因而更适合于许多热敏性材料。 将藻油DHA转化为方便在工业化应用的DHA营养强化剂。因此选择乳化喷雾干燥法DHA微胶囊。

DB-FFAP 30m×0.25mm×0.25μm（P/N 122-3232） 进样口温度： 200℃ 载气： 氮气 1ml/min ； 分流比 10：1 柱箱温度： 50℃维持3min.，以25℃/min升温到170℃，保持1min FID检测器温度： 200℃ 氢气流速： 35ml/min 空气流速： 400ml/min 补偿气流速： 25ml/min

系统： ACQUITY UPLC® 色谱柱： ACQUITY UPLC HSS T3, 1.8 μm, 2.1×100 mm 柱温： 30 ?C 样品温度： 4 ?C 流速： 0.45 mL/min 流动相A： 水(0.1%甲酸) 流动相B： 甲醇(0.1%甲酸) 弱洗针液： 3:1:1水/甲醇/乙腈(1000 μL) 强洗针液： 5:1:1乙腈/异丙醇/水(500 μL) 梯度运行时间： 7.5 min 进样体积： 2 μL，满环进样

本文利用Labthink兰光XLW智能电子拉力试验机测试月饼塑料复合膜包装的拉断力与断裂伸长率，并介绍了试验原理、基本过程及试验设备的适用范围、参数等内容，可为企业在筛选监控包装耐油性时提供参考方法。