纳谱分析NanoChrom纳谱分析技术(苏州)有限公司NanoChrom Technologies (Suzhou) Co., Ltdwww.nanochrom.com 半胱氨酸偶联单抗药物的分离 Time [min] Column: BioCore HIC-Butyl, 5 pm Dimension: 4.6x100 mm Mobile phase B: 100 mM phosphate buffer, pH7.0 Mobile phase C: Isopropanol Gradient: t (min) %A %B %C -10 0 0 0 0 0 0 Flow rate: 1.0 mL/min Temperature: 30℃ Column: BioCore HIC-Butyl, 5 μmDimension: 4.6x100 mmMobile phase A: 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0Mobile phase B: 100 mM phosphate buffer, pH7.0Mobile phase C: IsopropanolGradient: t (min) %A %B %C -10 75 25 0 0 75 25 0 1 75 25 0 15 0 75 25 20 0 75 25Flow rate: 1.0 mL/minTemperature: 30 ℃Injection: 10 μLDetection: UV 280 nmSample: Cysteine conjugated ADC (~1 mg/mL in mobile phase A)