HPLC DATA 'SHEET Commercially available reagent(0.5, 0.17 mg/mL, correspond to standard solutionfor butenafine hydrochloride cream) mAU 1. 1 600- 500- Diphenyl(I.S.) 400- 2 300 2. 200 100- 0 R100826A8 Column : YMC-Pack Pro C8 (3 um, 12nm)50 ×3.0 mml.D. Eluent : 1 mM CH3COONH4/acetonitrile (1/4)Flow rate : 0.7 mL/min (adjust the flow rate so that the retention time of butenafine is about 2.5 min)Temperature : 40℃Detection : UV at 282 nm Injection :5pL (日本薬局方収載原案記載条件) 色谱条件及色谱柱规格见谱图