土壤中有机污染物检测方案

www.appliedseparations.com SFE Application Extraction of PAHs from Soil Introduction Supercritical CO2 aloneeasily dissolvespolyaromatichydrocarbons (PAHs)from spiked matrices， butnot from weathered， agedsamples. The addition of asmall amount of co-solvent is necessary todisplace the PAHs fromthe surface of a weatheredsample for complete extraction. The addition of a fixed quantity of methanol tostandard reference material prior to supercritical CO2extraction shows increased recoveries of PAHs whencompared to US EPA liquid/solid extractiontechniques. Equipment Applied Separations’Spe-ed SFE or HelixSupercritical Extraction System Materials Standard Reference Materia (PAHs in Soil)SFE grade CO2 C18 Collection cartridge(1g/6mL #12007) √ Pesticide grade methanol #511 Method Place 0.5g of soil/sediment into a 1mL extractionvessel and add 100pL of methanol. Extract sample atspecified conditions. Extraction Conditions Extraction vessel： 1mL Sample： 0.5g Pressure： 7000 psi Temperature： 80℃COz Flow Rate： 2L/min (gas)Collection： SPE cartridge 1g/6mL C18Static： 10 minutesDynamic： 30 minutes SPE Rinse： 5mL methanol spiked with internal standard Results SFE Recoveries of PAHs from Soil Certified bySoxhlet Certified SFE (mg/kg) (mg/kg) Naphthalene 32 31 Acenaphthene 19 17 Phenanthrene 1618 1573 Anthracene 422 493 Benzo(bk)flouranthene 152 180 Analysis GC-MS Conclusion A fixed quantity of co-solvent addition to a soilsample prior to extraction gave excellent results with100uL of methanol added. Only small quantities ofmethanol are required to displace the PAH analytesfrom the soil matrix and do not desorb the analytefrom the solid phase extraction cartridge used to trapthe analyte. 930 Hamilton Street