动物肉制品中脂肪检测方案

Abstract PSE Sample Preparation The Extraction of Fat from Frankfurtersand Roasted Chicken Breast bvPressurized Solvent Extraction Al KaziunasRobert MaxwellKathy Pearl Applied Separations 930 Hamilton Street Allentown， PA18101 www.appliedseparations.com Recently， alternative techniques for fatdetermination have been introduced. One of thesemethods is the Pressurized Solvent Extraction(PSE)) of meat products. This technique rapidlyextracts fat with minimal amounts of solvent andsignificantly reduces manpower requirements andlab analysis costs. AAppliedSeparations Abstract， cont Fat extractions by PSE were conducted ondifferent samples， including frankfurters， androasted chicken breast， and were compared to thestandard petroleum ether extraction technique.Results including the correlation of the PSE andthe reference solvent extraction method for highfat frankfurters and low fat roasted chicken breastare presented. AppliedSeparations Introduction Pressurized solvent extraction (PSE) is a new sampleextraction technique that reduces solvent consumptionand sample preparation time. Solvent is pumped intoan extraction vessel that contains the sample to beextracted.The vessel is heated andpressurized.Thepressurized solvent at high temperature accelerates theextraction of analytes by increasing analyte solubilityand also increasing the kinetic rate of desorption ofthe analyte from the sample matrix. AppliedSeparations Introduction， cont. PSEcan be used to replace soxhlet extractions offat from various food samples. Current extractionmethods use significant quantities of organicsolvents， and require long extraction times. AAppliedSeparations The fast PSE The fast PSE is anautomated solventextraction instrumentcapable of six (6) simu taneous extractions， thereby increasing sample throughput six-fold over current systems. AppliedSeparations fast PSE Equipment： Specifications Instrument： fast PSE by FASTApplied SeparationsEASTPressure： 150 BARTemperature：150°CSimultaneousExtractionss：： 6SepaAprpaltiExtraction VesselieodnsCapacity： 11， 22，33mL AppliedSeparations one PSE The one PSE is anautomated solventextraction instrument withall the features of the fastPSE， except that it runsone sample at a time. 三AppliedSeparations one PSE EquipmenttSSpecifications nstrument： one PSE byApplied SeparationsPressure： 150 BARUTemperature：150°CExtraction VesselPSECapacity： 11，22，33mL Grind 1g meat product with 2g Spe-ed MatrixMicrowave 4 minutes #Pack sample in extraction vessel AppliedSeparations PSE Extraction Conditions Temperature： 125°C Pressure： 100 bar ： Static time： 1 minute Number refills： 7 t Refill volume： 2mLs wFlush program： 20 seconds/2 minutes/0 Sample size： 1g Solvent： Petroleum ether AppliedSeparations Gravimetric Analysis Fat Content%=1100 (B-C) A A=Sample weight B=Weight of flask after extraction C=Weight of flask prior to extraction AAppliedSeparations Standard Method Soxhlet Extraction AOAC 945：16 Grind sample with sand in thimble and dry for 2hours Extract in Soxhlet apparatus for 6 hours AppliedSeparations PSE Resu tsVSSoxhlet AppliedSeparations Comparison of Extractionl Timesand Solvent Consumption AppliedSeparations Sample processing time is reduced from hoursto minutes Solvent consumption is reduced significantlyExtraction results compared closely to soxhletvalues for high fat and low fat samples AppliedSeparations