糖的HPLC-ELSD分析

蒸发光散射检测器（ELSD）是一种通用的HPLC检测器，其检测原理基于光散射现象。在使用热去除挥发性流动相并用氮气喷射之后，当光照射到残留的非挥发性组分的颗粒上时发生散射。在检测部分中，LED被用作照射非易失性组件的光源，并且散射光被光电倍增器转换为电信号以测量强度。糖和脂肪通常使用折射率检测器或紫外线检测器的短波长范围进行测量，可以以更高的灵敏度和更稳定的基线进行测量。在本报告中，单糖和双糖在HILIC模式下使用ELSD和聚合物NH2柱进行分析。2/3Analysis of Sugars by HPLC-ELSDApplication Note Application Not e JS.C O Analysis of Sugars by HPLC-ELSD An evaporative light scattering detector (ELSD) is a universal HPLC detector whose detection principle is based the light scattering phenomenon. The scattering occurs when light is i rradiated on to particles of residual non-volatile components after removing the volatile mobile phase using heat and sprayed with nitrogen gas. In detection portion， LED is used as the light source for irradiating the non-volatile components and scattered light i s converted into an elect r ical signal by the photomultiplier to measure the intensity. Sugar and fat， which had been usually measured using a refractive index detector or a short wavelength range of a UV detector， can be measured with higher sensitivity and a more stable baseline .In this report， monosaccharides and disaccharides were analyzed using ELSD with a polymer NH， column in HILIC mode. PU-2089 Keywords： Sugar， HILIC， Polymer NH， column， ELSD，Rhamnose， Fructose， Glucose， Sucrose， Maltose Equipment Condit i ons Pump： PU-2089 Autosampler： AS-2057 Column oven： CO-2060 Detector： ELS-2040 Shodex Asahipak NH2P-50 4E (4.6 mmlDx250 mmL) Eluent A： Water/Acetonitrile (25/75) Flow rate： 1.0 mL/min Column temp.： 30°℃ FELSD condition： Nebulizer temp.： 30℃Evaporator temp.： 30℃Gas flow rate； 1.4 SLM Injection volume： 10pL Standard sample： Rhamnose， Fructose， Glucose， Sucrose， Maltose 1.0 mg/mL each in Water/ Acetonitrile (50/50) Figure 1 shows the chromatogram of the standard mixture of monosacchar i des and disaccharides . Figure 1. Chromatogram of a standard mixture of sugars. 1： Rhamnose，2： Fructose， 3： Glucose，4： Sucrose， 5： Maltose