蛋白质中不同浓度下黏度变化检测方案(粘度计)

京都電子工業株式会社KYOTO ELECTRONICSMANUFACTURING CO.，LTD.APVS-0109en-1 APVS-0109en-16. Procedure Application Note Concentration dependence of viscosity of protein solutions IndustryPharmaceutical，Food & beverageInstrumentViscometerMeasurement method：Electro Magnetically Spinning MethodStandards 1.Scope Albumin is one of the most widely used proteins in the pharmaceutical industry. Bovine Serum Albumin (BSA) is an essential ingredient for the production of therapeutic drugsand extracorporeal diagnostic agents as enzyme stabilizers and the like. Examples of measuring the concentration dependency of the dynamic viscosity of proteinsolutions using an EMS viscometer that can be measured by sealing， sterilization andnon-contact were shown below. 2.Precautions None. 3.Post-measurement procedure The sample container and the sample are discarded appropriately. 4. Apparatus EMS Viscometer Control Laptop PC 5.Reagents ·Sample： Bovine serum albumin， ovalbumin， " Phosphate Buffered Saline (PBS(-)) solution 京都电子工业株式会社-可睦电子(上海)商贸有限公司 上海市徐汇区宜山路333号1201室电话：021-54488867 电邮： kemu-kem@163.com 网址： http：//www.kem-china. com 1) Enter the following conditions in measurement condition of the sequence mode of controlsoftware. Measurement mode ：Sequence mode Measurement temperature ：25℃ Motor rotation speed ：1，000 rpm Measurement time ：I (1 second) Repeat count ：5 times Measurement interval ：5 seconds Waiting time for temperature stability ：5 minutes 2) Place an aluminum spherical probe ofp 2 mm and a sample of 300 uL in a container， coverwith a cap and packing， set the sample container in the EMS Viscometer， and click themeasurement button. 3) Add the dilution solvent after the first measurement is completed， and measure in sequence underthe same condition. 7. Example The concentration dependence of viscosity of bovine serum albumin and ovalbumin both can beconfirmed. The measurement results are shown in Figure 1 and Table 1， 2. The viscosity ofbovine serum albumin solutions is obtained higher value compared with that of ovalbuminsolutions at high concentrations of 250 mg/mL or more， though a significant difference ofviscosity between two kinds of protein solutions at low concentrations is not observed. Figure 1. Measurement result about concentration dependence of viscosity of protein solutions京都电子工业株式会社-可睦电子(上海)商贸有限公司 -上海市徐汇区宜山路333号1201室电话：021-54488867 电邮：kemu-kem@163. com 网址： http：//www.kem-china.com Table 1. Concentration dependence of viscosity of bovine serum albumin (mPa's) Concentration (mg/mL) 50 100 150 200 250 300 350 1st 1.53 1.61 2.31 2.82 4.27 6.56 11.30 2nd 1.53 1.61 2.31 2.82 4.26 6.55 11.40 3rd 1.53 1.61 2.31 2.81 4.26 6.57 11.30 4th 1.54 1.62 2.29 2.82 4.27 6.63 11.30 5th 1.53 1.62 2.33 2.82 4.27 6.57 11.30 Average 1.53 1.61 2.31 2.82 4.27 6.58 11.32 Standard deviation 0.00 0.01 0.01 0.00 0.01 0.03 0.04 RSD 0.3% 0.3% 0.6% 0.2% 0.1% 0.5% 0.4% Table 2. Concentration dependence of viscosity of ovalbumin (mPa's) Concentration (mg/mL) 50 100 150 200 250 300 350 lst 1.30 1.60 1.94 2.79 3.72 5.96 8.35 2nd 1.29 1.60 1.92 2.73 3.73 5.98 8.34 3rd 1.29 1.60 1.92 2.73 3.73 6.04 8.38 4th 1.30 1.60 1.92 2.75 3.73 5.98 8.42 5th 1.29 1.59 1.92 2.75 3.73 5.99 8.32 Average 1.29 1.60 1.92 2.75 3.73 5.99 8.36 Standard deviation 0.01 0.00 0.01 0.03 0.00 0.03 0.04 RSD 0.4% 0.3% 0.5% 1.0% 0.1% 0.5% 0.5% 8. Summary Even if it is a rare sample (expensive sample) such as the protein， it is possible to evaluate theconcentration dependence with a small amount of sample by measuring while adding thedilution solvent and diluting. 9. References None. 都電子工業株式会社KYOTO ELECTRONICSMANUFACTURING CO.，LTD 蛋白质溶液在不同浓度下黏度变化的研究 白蛋白是制药工业中应用最广泛的蛋白质之一。牛血清白蛋白(BSA)是生产治疗药物和体外诊断剂作为酶稳定剂等的重要成分。使用EMS粘度计测量蛋白质溶液在不同浓度下的粘度变化，可以通过密封、灭菌和非接触来测量。即使是稀有的样品或昂贵的样品，如蛋白质，也可以通过在样品中加入稀释溶剂来测量少量样品的粘度值。