番茄副产物中提取番茄红素检测方案(超临界萃取)

The Supercritical CarbonDioxide Extraction ofLycopenes from TomatoBy-products Al Kaziunas Applied Separations 930 Hamilton Street Allentown， PA\1188101 Applied Separations www.appliedseparations.com Abstract Tomatoes contain a highconcentration of carotenoids andlycopenes， significant amounts ofwhich are found in the skins of thetomatoes. Lycopenes are valuedntrfor their significant antioxidantproperties， and are reported toprevent cardiovascular disease. Abstract.t. cont. Methods are needed to extractlycopenes from natural sources foranalytical purposes and also naturalprocessing. This paper investigatesthe use of supercritical carbon dioxideto extract lycopenes from tomato skinsas a non-toxic alternative to traditionalorganic solvent extraction methods. Abstract.. cont. Supercritical extraction parameters wereoptimized by varying the carbondioxide density. Extraction pressureswere varied between 200 and 680BAR and temperatures from 40°C to80°C to accomplish density changesand determine optimal extractionconditions. Details of samplepreparation， supercritical extractionand lycopene analysis are presented. ntroduction Lycopene is a carotenoid compound found inhigh concentrations in tomatoes， and is thecompound that gives them the red color. Inaddition， lycopenes are reported to haveantioxidant， anti-carcinogenic， and othermedicinal properties. The solvent extraction of lycopenes is a timeconsuming process that requires largevolumes of organic solvents. This poster demonstrates the use ofsupercritical CO， as a solvent-free alternativeto the solvent extraction of lycopenes fromtomatoes. AppliecSeparations LycopeneStructure H，C. CH H CH CH CH CH CH， HC CH， Applied Separations B-Carotene ：Structure H，C CH CH CH CH， C CH CH， CH Instrument SpecificationsSpe-ed SFE-NP Pressure 10，000 psi (680 BAR) lemperature 250°C CO， Flow Rate 50L/min (expandedgas) Extraction Vessel 1 liter AppliedSeparations Spe-ed SFE-NP Sample Preparation 12 lbs of whole， ripe tomatoeswere cut into quarters Tomato solids were separatedfrom liquid by crushing thetomatoes in cheesecloth andretaining the solids Tomato solids were dried in avacuum oven for 24 hours at 40°Cand ground in a laboratory mill Optimized Supercritical CO2Extraction Conditions Sample 30 grams Extraction vessel 50mL Pressure 680 BAR Temperature 80°C Flow rate 4 L/min.， CO， asGas Collection 60mL， tared amber vial Analysis gravimetric Applied Separations Supercritical ExtractionProfile Note： 100% of extractables is based on soxhletextraction results Comparison of ExtractionMethods Solvent Extraction Soxhlet Supercritical CO，2 Solvent Acetone/Hexane(1：1) CO， Extraction Time 10 hours %4hour Organic SolventConsumption 200mL 0 Recovery (%soxhlet) 100% 96.1% AppliecSeparations Conclusion Supercritical CO， extraction oflycopenes from tomatoes is： -Fast -Eliminates toxic organic solvent -Comparable to soxhlet extractionresu ts References Khacik F.， J. Agric FoodChem 1992， 40， 390-398. Favati F.， Fourth Italian Conference onSupercritical Fluids and Their Applications，1997，121-128.