(1)台式机尺寸的紧凑设计!可安装于HPLC实验室;可使用AC220 V电源节省空间、紧凑的设计,实现了与HPLC系统相同的安装面积;成功将N2气体使用量控制在最小限度(最大流量3.0 L/min)。也可使用N2气瓶。可与目前使用的HPLC进行连接(详情请向销售工程师咨询) (2)与HPLC检测器具有同等的操作性 仪器/方法的设定 为了使初次使用者都能进行质谱分析,避免了复杂的设定操作,实现了更为简单的操作性。 另外,针对HPLC用户感觉复杂的调谐,通过自动调谐功能,可轻松获得最佳仪器状态。 测量结果 通过等高线显示等可对整体的色谱洗脱模式进行确认。 与HPLC的DAD(二极管阵列)检测器的解析画面非常相似且简单易懂的操作画面,实现了与一般HPLC检测器相同的操作性。 (3)与HPLC检测器具有同等的维护性 当灵敏度下降及怀疑有污染物时,拆下大气压离子过滤器,可轻松进行清洗。 而且在拆下进行清洗时,无需停止真空泵,因此,进行维护后可顺畅地开始测量。
HITACHIInspire the NextTechnical ReportAS/MSD-004 High Performance Liquid Chromatographyhttp://www.hitachi-hitec-science.comC2015 Hitachi High-Tech Science Corporation Analysis of Methylxanthines by PDA Detector and Mass Detector Chromaster 5610 MS detector is a new mass detector, designed for LC users, and it isdifferent from conventional mass spectrometers. This time, the analysis examples ofmethylxanthines such as caffeine are introduced here. These components are oftencontained in coffee, cacao products, and supplement drinks. Methylxanthines have acommon basic molecular structure. Therefore, UV spectral patterns are very similar andthe differentiation based only on the UV spectra is difficult. However, a mass detector canprovide the mass information unique to a component, and thus the identificationaccuracy is improved. 5610 MS Detector LC-MS measurement of Methylxanthines series 4 compounds ■ Analytical Conditions Table 2 HPLC Xonsiriona Table 1 MS Detector Setting Conditions lonization method ESI lonization mode Positive lonization voltage 2800V Measurement mode Scan (m/z 100-300) Column LaChrom Ⅱ C18 (3 um), 4.6 mm I.D. x100 mm Mobile phase A: 0.1%formic acid solution B: 0.1%formic acid solution-Methanol solution B10%(0min)→B50%(5min)→B10%(5.1-8min) Flow rate 1.0mL/min (250:1 sprit) Column Temp. 50°C Detection w.l. DAD 275 nm Injection Volume 10uL ■LC-MS Measurement Each compound, Caffeine, Theobromine, Theophylline and 7-(2-hydroxyethyl)-Theophylline, is dissolvedand diluted with methanol so as to prepare the sample at concentration of 10 p g/mL. Due to the similarchemical structure, UV spectra obtained by DAD have a similarity. However, MS Detector permits to identifythe compounds by the confirmation of [M +H]+ion. 2 3 Detection: DAD 275 nm 2 3 Detection: MSD (Scan) 0 1 2 3 4 5 6 7 8 Time (min) 4.7-(2-Hydroxyethyl)- 1.Theobromine 2. Theophylline 3. Caffeine theophylline Fig. 1 Measurement result of DAD and MS Detector Main system configuration: 5110 Pump, 5210 Autosampler, 5310 Column Oven, 5430 DAD, 5610 MS DetectorNOTE: These data are an example of measurement; the individual values cannot be guaranteed. @Hitachi High-Tech Science Corporation
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