（1）利用电磁阀切换频率加倍的功能，chromaster引进了一种新型的低压梯度系统——高频模式（High Frequent Mode）。HFM与日立独有的 “高速反馈实时控制”系统相配合，大大抑制液体脉冲，提高了梯度精度和保留时间的重现性。 （2）依靠配置的高精度注射器驱动单元，实现卓越的进样精度。 日立极大地降低了自动进样器流路内引起残留的死体积，并采用清洗外壁的泵，实现了极低的样品残留。 （3）紫外/紫外可见检测器 双波长高灵敏度检测，特别针对药物有关物质检查。双波长检测功能可在更短的数据采集间隔条件下实现，每个波长数据采集间隔可设定为800ms，甚至400ms，可以得到更好、更尖锐的峰形。 二极管阵列检测器 宽波长范围（190～900nm）和高光谱分辨率（配1024bit二极管）的chromaster二极管阵列检测器致力于打造世界上最高水平的高分辨率分析。 更低噪音 二极管阵列检测器噪音值和紫外检测器相当，支持高灵敏度分析。
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HITACHITechnical Report1nspire the NextAS/LC-054 High-Performance Liquid ChromatographAnalysis of D-Mannitol in Japanese Pharmacopoeia Sixteenth Edition Supplement Ilhttp：//www.hitachi-hitec-science.comC2015 Hitachi High-Tech Science Corporation The test items for D-mannitol in the Japanese Pharmacopoeia Sixteenth Edition Supplement ll， whichwas put into force in February 2014， have been revised， and the analysis by HPLC has been added.For D-mannitol， the analysis for Purity (4) Related Substances and Quantitative Method is performedat 85°C with a 300 mm column， and thus a large column capacity as well as the temperature control toensure stable analysis even at a high temperature will be required.Chromaster 6310 Column Ovencan store a maximum of three 300 mm columns. It provides a wide temperature setting range of 4-90°C and excellent temperature stability and is capable for the low volume preheating to reduce thepeak diffusion. As a result， the system can be used for various applications. This time， the analysis ofD-mannitol was performed under the conditions for Quantitative Method described in the JapanesePharmacopoeia Sixteenth Edition Supplement Il and the analysis is introduced here. High Performance LiquidChromatograph Chromaster@ Quantitative Method of D-Mannitol- Confirmation of System Suitability Standard solution： 0.5 g of D-mannitol is dissolved in purified water， and the volume is made up to 10mL (5%). System suitability test solution (1)： 0.25 g of D-mannitol and 0.25 g of D-sorbitol are dissolved in purified water， and the volume ismade up to 10 mL (2.5% each). System suitability test solution (2)：0.5 g of maltitol and 0.5 g of isomalt are dissolved in purified water， and the volume is made up to100 mL (0.5% each). Purified water is added to 2 mL of this solution to make the volume to 10 mL (0.1% each). Table 2 shows the result of the system suitability test for the Quantitative Method of D-mannitol. Results satisfying the specificationswere obtained for all the items. Figure 1 Chromatogram of System Suitability Test Solution (1) Figure 2 Chromatogram of System Suitability Test Solution (2) Table 1 Analytical Conditions Figure 3 Chromatogram of Standard Solution Column(Transgenomic) CARBOsep COREGEL-87C7.8 mm l.D. X 300 mm Mobile phase H20 Flowrate 0.5 mL/min Column temperature 85°C Detector RI (Cell temperature： 40℃) Injection vol. 20 pL Table 2 Quantitative Method of D-Mannitol -System Suitability Test Result Item Specificationvalue Result System performance (relative retentiontime) (Figure 1， Figure 2) D-Mannitol-Isomalt (1)D-Mannitol-Maltitol D-Mannitol-Isomalt (2) D-Mannitol-D-Sorbitol Approx.0.6Approx.0.69Approx. 0.73Approx. 1.2 0.630.690.731.23 System performance (resolution)(Figure 1) D-Mannitol-D-Sorbitol NLT 2.0 5.74 System reproducibility (Figure 3) D-Mannitol Relative standard deviation (n=6) of peak area (%) NMT 1.0 0.21 Chromaster 5110 Pump， 5280 Autosampler， 6310 Column Oven， 5450 RI Detector NOTE： These data are an example of measurement； the individual values cannot be guaranteed. @Hitachi High-Tech Science Corporation
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日立科学仪器（北京）有限公司为您提供《D-甘露醇中有关物质检测方案(液相色谱仪)》，该方案主要用于原料药中限度检查检测，参考标准--，《D-甘露醇中有关物质检测方案(液相色谱仪)》用到的仪器有日立Chromaster高效液相色谱仪
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