HPLC DATA'SHEET CHCOOH Column : YMC-Pack ODS-A (10um, 12nm)300x4.0 mml.D.Eluent :phosphate buffer (pH 2.8)*/ acetonitrile (80/20)※Dissolve 8.57 g of NH4H2PO4 and 1 mL of H3PO4 in 1000 mL waterFlow rate : 1.3 mL/min(adjust the flow rate so that the retention time of chlorphrniramine maleate is about 11 min)Temperature : 25°CDetection : UV at 225 nmInjection :20 uL (0.003 mg/mL) 色谱条件见谱图