阿昔洛韦中鸟嘌呤检测方案(液相色谱柱)

HPLCDATA 'SHEET 了沙夕口匕儿(第十五改正日本薬局方第二追補記載条件)Aciclovir (The Japanese Pharmacopoeia 15th Edition Supplement Ⅱ) R1007130 Purity standard solution (Guanine 0.005 mg/mL) 1. mAU 9 HNOH 30： H2N N Aciclovir 2 2. R100713D Purity sample solution (Aciclovir 1.0 mg/mL) Guanine mAU R100713E Column ： YMC-Pack ODS-A (3 um， 30 nm) 100X4.6 mml.D. Eluent ： phosphate buffer containing 1-decansulfonic acid sodium salt/acetonitrile (1000/40) ※Dissolve 1.0 g 1-decansulfonic acid sodium salt and 6.0 g NaH2PO4·2H2O Flow rate in 1000 mL water → adjust pH 3.0 by H3PO4 ： 1.4 mL/min Temperature ：20c Detection ： UV at 254 nm Injection ：10 pL (第十五改正日本薬局方第二追補記載条件) 色谱条件见谱图。