尿液中铜元素的分析

Aurora Instruments Ltd.Application Notice AA-2For more details， please contact：Aurora Instruments Ltd.1001 East Pender St.， Vancouver B.C.Canada， V6A 1W2 Application Procedure for Analyzing Copper in Urine Sample Preparation Procedure： 1. Thaw the urine samples at room temperature for 15-20 minutes. 2. Homogenize the samples by slowly vortexing. 3. Centrifuge at 14400 rpm for 2-3 minutes. 4. Dilute the urine samples with pure deionized water in a 1：3 ratio into a microplate (For example，80pL urine +240uL water). 5. Homogenize the diluted samples by mixing them on a microplate mixer for 2-3 minutes. Standard Preparation： The standards for the calibration curve are to be made up from a known concentration of NIST#2670 elevated concentration Reference Standards. Standard No. NIST standard volume (uL) Watervolume (uL) Concentration (ppb) 1 2.5 317.5 2.9 2 5.0 315 5.8 3 10 310 11.6 4 20 300 23.2 5 30 290 34.8 Operating Conditions： Instrumentation： Aurora Al 1100/2100 GFAAS. Parameter of settings： Injection Volume-20pL AURORA INSTRUMENTS AI 1100/2100 Method Name Urine Element Name Cu Instrument Mode Absorbance Display Mode Corrected Manual Band Pass 0.7nm Lamp 1 Current 3.0 mA Lamp 2 Current 0.0 mA Wavelength 327.36nm PMT Voltage 301.0V Preheat Steps 0 Cooling Time 60s Inject Speed 5 Furnace Profile Steps 8 Cont. on back Step finaltemp ramptime holdtime GasFlow PlasmaON CollectData Integrate 1 50 0.00 1.00 1.50 Off Off Off 2 130 12.00 1.00 1.50 Off Off Off 3 300 6.00 1.00 1.50 Off Off Off 4 850 10.00 2.00 1.50 Off Off Off 5 850 0.00 4.00 1.50 Off On Off 6 2300 0.00 1.00 0.50 Off On Off 7 30 0.00 3.00 1.50 Off On Off 8 30 0.00 18.00 1.50 Off Off Off Tel： (604) 215-8700Fax： (604)215-9700 ( Web site： h ttp：//www.aurora-instr.com ) E-mail： aurora@intergate.bc.ca