大豆卵磷脂中主要成分分析检测方案(液相色谱柱)

HPLC Conditions ba060 Column ：CAPCELL PAK Ca MGII S5 ：4.6 mm i.d.x 250 mm Mobile phase ： CH：0H Flow rate ： 1.0 mL/min Temperature ： 40℃ Detection ： RI Inj. vol. ： 20 pL Pretreatment ： The standard compounds were dissolved in chloroform at 10 mg/ mL， and diluted to 1 pg/mL with methanol. Soymilk was diluted5-fold with methanol， and then shaked for 30 seconds. After thesolution was centrifuged (1500 rpm) for 30 seconds， the upperlayer was filtered with a 0.2-pm filter and introduced to HPLC※1 pg/mL=1 ppm