大豆异黄酮中主要成分分析检测方案(液相色谱柱)

HPLC Conditions ba058 Column ： CAPCELL PAK Ca MGII S5： 4.6 mm i.d. x250 mm Mobile phase ： Trifluoroacetic acid was added to a solution (water / methanol =10 / 90) at 0.1 vol% Flow rate ：1.0mL/min Temperature ：40℃ Detection ： UV 215 nm Inj. vol. ：5pL Pretreatment ： The standard compounds were dissoled in methanol at 1 pg/mL. Soymilk wasdiluted 5-fold with methanol， and then shaked for 30 seconds. After the solutionwas centifuged (1500 pm) for 30 seconds， the upper layer was filtered with a0.2-pm flter and introduced to HPLC ※ 1 pg/mL=1 ppm