其他检测

对生物过滤器的挑战实验是向带正电的过滤器进水中不断注入高含量的内毒素，然后采用鲎变形细胞溶解物试验（动态浊度法）测量产水中的内毒素浓度。大多数的内毒素挑战实验均要使用净化的LPS。 ELGA LabWater的科研团队从纯水中已有的细菌中生产出自己的LPS。这是为了模仿逼真的挑战实验环境。

本文介绍了SupelMIP分子印记SPE小柱选择性提取b-受体激动剂和拮抗剂。 关于Supelco 美国Supelco公司成立于1966年，一直致力于色谱耗材的研究和生产，是色谱耗材的专业生产公司。超过40年在色谱和分析领域的技术经验，拥有多项专利技术，提供范围广泛的产品：气相色谱柱（包括手性柱）和配件、液相色谱柱（包括手性柱）和配件、固相萃取小柱和装置、固相微萃取手柄和萃取头、空气检测产品、分析标准品和样品瓶等。

介绍： CyBi-SELMA（CyBio品牌，德国耶拿公司）半自动移液工作站以其体积小、操作界面友好、兼容96或384孔板的特点，在低通量液体处理操作中非常适用。 由于便于放入组织培养生物安全柜中，此设备非常适合在基于细胞的研究实验中应用，例如培养基更换、化合物浓度梯度筛选。 iPSC制备的心肌细胞（iCell Cardiomyocytes）被《科学家》网站评选为2010年度生命科学领域的十大创新产品。位于美国威斯康星州麦迪逊市的Cellular Dynamics International (CDI)公司的研究人员将人类纤维细胞诱导生成多功能干细胞(iPSC)后，进一步对iPSC细胞进行重编程获得了人类心肌细胞产品iCell Cardiomyocytes，该细胞显示了活的心脏典型的电生理特征。这是目前第 一个商业化的人类干细胞分化细胞系。iCell Cardiomyocytes为研究人员提供了最方便的细胞类型进行相关的特异性研究。此产品的主要目的是用于药物发现。药物毒性是药物研发中的一个严重问题，是药物退出市场的第二大原因。 如何使药物更安全，甚至保全生命，是药物研发领域的很大机遇。 Chris Kendrick-Parker还介绍说，CDI公司每天都会制造、销售出数十亿的心肌细胞产品，在全球顶尖的20家制药公司中已经有一半的公司都成为了CDI公司的客户。 这类人心肌细胞具有广泛用途，包括用于药物活性成分的心脏毒性研究。这种检测中的移液操作包括梯度稀释、化合物添加、细胞液添加，都是乏味、耗时，并且受到不同操作者在技术水平、准确度、重现性差异的影响。 本实验采用Promega公司的CellTiter-Glo® Luminescent Cell ViabilityAssay(CellTiter-Glo®发光法细胞活力检测试剂盒)。是通过对ATP 进行定量测定来检测培养物中活细胞数目的一种均质检测方法。ATP是活细胞新陈代谢的一个指标。CellTiter-Glo®检测试剂盒为多孔板而设计，是进行自动化高通量筛选(HTS)、细胞增殖和毒性分析的理想选择。均质检测步骤就是将单一试剂(CellTiter-Glo®试剂) 直接加入含有血清的培养细胞中，无需洗涤细胞、去除培养基或进行多步加样操作。在384 孔板上，加入试剂并混合后，10分钟内，该系统可检测到的每个孔内的细胞数最低为15 个。 CyBi-SELMA用于一系列化合物的心脏毒性研究实验。对于SELMA自动化设备相比人工移液的操作友好性、快速、数据一致性进行评估。

Biochrom 30+系列提供的氧化蛋白水解程序作为标准程序，当客户由于环境，试剂改变，或者分离柱老化，可能需要稍做改动以达到在氧化蛋白水解标准中的最佳氨基酸分离，为此我们提供优化方法给客户以方便大家得到更好的结果。

摘要： 微波辅助萃取技术首次被用于黄芪黄酮的提取。研究考察了微波功率、提取次数、乙醇浓度、提取温度、提取时间以及固液比几个影响微波提取得率的参数。得出在乙醇浓度为90%、提取温度110 oC、提取时间25 min以及固液比25 ml/g时取得**提取率。在优化提取条件下没有观察到黄酮的降解。最有提取得率为1.190 ± 0.042 mg/g，与甲醇索氏提取30 min两次得率相近，较传统90%乙醇回流提取2h两次得率高。

"应用日本京都电子公司(KEM)[AT-510]自动电位滴定仪酸碱滴定测试水解蛋白中的甲醛氮_En 的应用资料实例En 。 适用标准 "

人体对由不锈钢或钛制成的植入材料的接受程度依赖于材 料最表层的生物相容性。不同表面的性质决定它的生物相 容性。表面电荷带来的静电作用力对蛋白的吸引作用是骨 融合的前提条件，例如牙移植。另一方面，预期的静电排 斥作用阻止了能引发感染的蛋白基团的吸附。 因此表面知识对开发和认证用于移植的生物材料最为重 要。

晶体学和NMR是进行生物大分子结构分析的惯用方法。 小角X-射线散射（SAXS）与这两种可提供高分辨有价值信息的方法具有 互补作用。与晶体学相比，SAXS具有独特的优点：SAXS可以获得溶液中 蛋白质的三维结构和蛋白质的聚集情况，这对更好的理解蛋白质的生物功 能是必需的。 由从头计算法得到的低分辨三维结构是对由蛋白质晶体学和NMR得到的高 分辨结构不可缺少的补充信息。

利用具有自学习特点的人工神经网络可实现对酶解过程的模拟仿真，研究从合浦珠母贝肉中制备抗菌肽的最佳工艺条件。采用3 层(5-9-3)人工神经网络法对风味蛋白酶水解合浦珠母贝肉的工艺过程进行模拟和优化，并通过管碟抑菌法对产物的抑菌性质进行分析。结果表明：pH7.0、水解温度55℃、酶添加量1.6%、水解时间4h、料液比7:5，制备得到肽A，抑制鼠伤寒沙门氏菌最强，抑菌圈直径14.20mm，平均肽链长度2.6；pH7.0、水解温度55℃、酶添加量1.7%、水解时间4h、料液比3:2，制备得到肽B，抑制痢疾志贺氏菌最强，抑菌圈直径23.42mm，平均肽链长度2.8；pH6.5、水解温度60℃、酶添加量2.5%、水解时间4h、料液比7:5，制备得到肽C，对单核细胞增生李斯特菌的抑制效果最强，抑菌圈直径16.60mm，平均肽链长度2.5。3 种抗菌肽对大肠杆菌、金黄色葡萄球菌也具有较强的抑菌效果，抑菌率为74.3%～80.8%。本研究利用人工神经网络优化制备的贝肉抗菌肽克服了纯度低、提取率低等缺点，为合浦珠母贝肉抗菌肽的开发利用提供技术支撑。

The objective of this project is to study the aerodynamics around a birdlike airfoil. The variables to investigate are the turbulent shear stress and the pressure distribution. With this aim, two experiments were carried out in the LNB wind tunnel. The seagull hand pinion inspired the SG04 birdlike airfoil considered for this work. A flapping flight motion apparatus was used to force this wing heaving and pitching. First, an experiment to capture the boundary layer and determine the shear stress was done. Therefore, very accurate measurements with stereoscopic Particle Image Velocimetry technique were required. This exercise was performed with a flexible and non-flexible SG04 wing. Moreover, with the same method, a second experiment tried to capture a larger flow field around the whole flexible flapping SG04 airfoil, in order to determine the pressure distribution. To begin with, a theoretical background highlighting the most important features of moving airfoils and Particle Image Velocimetry is presented. The experimental setup, recording, data processing and results of both experiments conclude the second part of this work.

本文采用简易的固相萃取及HPLC法分析血浆中儿茶酚胺。 关于Supelco 美国Supelco公司成立于1966年，一直致力于色谱耗材的研究和生产，是色谱耗材的专业生产公司。超过40年在色谱和分析领域的技术经验，拥有多项专利技术，提供范围广泛的产品：气相色谱柱（包括手性柱）和配件、液相色谱柱（包括手性柱）和配件、固相萃取小柱和装置、固相微萃取手柄和萃取头、空气检测产品、分析标准品和样品瓶等。

Aerodynamic theory and empirical observations of animals flying at similar Reynolds numbers (Re) predict that airflow over hummingbird wings will be dominated by a stable, attached leading edge vortex (LEV). In insects exhibiting similar kinematics, when the translational movement of the wing ceases (as at the end of the downstroke), the LEV is shed and lift production decreases until the energy of the LEV is re-captured in the subsequent half-cycle translation. We here show that while the hummingbird wing is strongly influenced by similar sharp-leading-edge aerodynamics, leading edge vorticity is inconsistent, varying from 0.7 to 26 per cent (mean 16%) of total lift production, is always generated within 3 mm of the dorsal surface of the wing, showing no retrograde (trailing to leading edge) flow, and does not increase from proximal to distal wing as would be expected with a conical vortex (class III LEV) described for hawkmoths. Further, the bound circulation is not shed as a vortex at the end of translation, but instead remains attached and persists after translation has ceased, augmented by the rotation (pronation, supination) of the wing that occurs between the wing-translation half-cycles. The result is a near-continuous lift production through wing turn-around, previously unknown in vertebrates, able to contribute to weight support as well as stability and control during hovering. Selection for a planform suited to creating this unique flow and nearly-uninterrupted lift production throughout the wingbeat cycle may help explain the relatively narrow hummingbird wing.

To date, wake measurements using particle image velocimetry (PIV) of bats in flight have studied only three bat species, all fruit and nectar feeders. In this study, we present the first wake structure analysis for an insectivorous bat. Tadarida brasiliensis, the Brazilian free-tailed bat, is an aerial hunter that annually migrates long distances and also differs strikingly from the previously investigated species morphologically. We compare the aerodynamics of T. brasiliensis with those of other, frugivorous bats and with common swifts, Apus apus, a bird with wing morphology, kinematics and flight ecology similar to that of these bats. The comparison reveals that, for the range of speeds evaluated, the cyclical pattern of aerodynamic forces associated with a wingbeat shows more similarities between T. brasiliensis and A. apus than between T. brasiliensis and other frugivorous bats.

In this work multimodal operation of commercially available CARS microspectrometer (EKSPLA CARSCOPE) equipped with a picosecond dual wavelength laser source (EKSPLA PT259) was demonstrated for the imaging of various biological objects using coherent anti‑Stokes Raman scattering (CARS), two photon excited fluorescence (TPEF) and second harmonic generation (SHG) contrast mechanisms. Obtained CARS‑images of yeast cell and flower pollen clearly demonstrate label‑free and 3D imaging capabilities of CARS technique. Multi‑contrast images of green algae and plants containing starch demonstrate a variety of research facilities, where the method can be applied.

THE NANOTEST VANTAGE: COMPREHENSIVE NANOMECHANICAL TESTING TO MIMIC IN-VIVO CONDITIONS FOR THE BIOMEDICAL SECTOR Hydrogels Functionaly graded materials Soft biological tissues Combinatorial testing Biomedical devices

MICRON SCALE INSECT ANATOMY The samples imaged were air-dried insects (ant, fruit fly, mosquito) and a tick (Arachnids). A number of structures were imaged to create a single classroom presentation in insect anatomy for North Shore Community College in Massachusetts, and as part of a broader series of classroom presentations directed at grades 9-12, physics, chemistry and biology, at Newburyport High School. The specimens were collected from readily available and indigenous species such as the tick, fruit fly, ant and mosquito. The images showed the students not only specific anatomical detail and inter specie variations but how detailed and complex these structures can be at very small scale.

拉曼光谱技术可以用来获取反映物质组成结构信息的指纹图谱。而表面增强拉曼（SERS）技术可以将通常非常微弱的拉曼信号强度放大到1百万倍以上。 Klarite芯片的独特设计和生产工艺使得它具有优秀的重现性，无论是医学诊断，药物检测 还是刑侦，国土安全等应用，Klarite芯片都可以一展身手。 产品特点 可重复性 -- 由于采用半导体制造工艺，获得了卓越的测试结果可重复性 灵敏度 -- 百万倍的信号增强 特异性 -- 保留丰富的固有拉曼信息 灵活性 -- 多种样品制备方法 应用 由于Klarite芯片所具有的高度可重复性。使得它可以被广泛应用于工业和科研应用中。 -- 国防和国土安全 （包括生化危险探测） -- 产品认证 -- 食品分析 -- 制药工业 -- 刑侦

活细胞成像技术是最近几年兴起的一项技术，能够在细胞接近生理的状态下观察细胞形态改变和蛋白的表达，该技术能够避免传统采用固定细胞或组织的研究方法中，固定细胞过程中造成的细胞形态的改变和结构改变，能够更加真实的反映出细胞的特性，因而广受推崇。MD ImageXpress Micor高内涵系统具有多种特性能够进行长达数天的活细胞的观察，同时，由于采用著名的MD软件产品MetaMorph为基础的MetaXpress高内涵分析软件，具有极高的灵活性和扩展能力，除活细胞成像之外还能够能够对某些细胞（如心肌细胞）的生理药理特性进行评价。

摘 要： 目的：考察微波辅助蛋白质水解效果的稳定性。方法：以鱼粉和豆粕为研究对象，采用微波辅助蛋白质水解-柱后衍生-紫外检测法测定氨基酸，通过比较分析一定时期内鱼粉和豆粕样品氨基酸结果的变化，研究微波辅助蛋白质水解效果的稳定性。结果：在一定时期内，采用微波辅助蛋白质水解，进口鱼粉、豆粕样品氨基酸测定结果RSD值均小于4.0%。结论：微波辅助蛋白质水解效果的稳定性好，可替代传统蛋白质酸水解方法，并为保证氨基酸检测结果的准确性提供技术支持。 关键词：氨基酸分析；微波辅助蛋白水解；稳定性

位于瑞士巴塞尔的罗氏药品开发中心，使用LabX软件与多台天平连接，这种完美的解决方案可以同时支持称量过程和满足可追溯文档系统的严格要求。

采用磁珠法的核酸提取工作站，与传统的核酸提取相比，省时省力，同时提高批处理能力和样品提起的一致性。避免用户接触生物毒性试剂。工作站性能稳定，一键式操作，方便快捷。

本文通过瑞士万通离子色谱仪，建立了离子色谱-电导检测器直接进样测定苏氨酸的方法。结果 最小检测限为1.8 mg/L；苏氨酸与其它阳离子同时测定时互不干扰；非离子态物质对苏氨酸的测定没有影响。离子色谱-电导检测器测定方法简单、准确，可用于苏氨酸的定量分析。

本文介绍了德国耶拿分析仪器股份公司生产的高速PCR的SpeedCycler2 的性能特点和应用。重点介绍了如何通过提高PCR 升降温速度，提高模块温度与样品溶液温度一致性，来极大地降低每个PCR循环的时间，从而达到提高整个PCR 反应速度的目的。指出了高速PCR提高PCR产物的特异性的可能性。同时也列举了高速PCR在人类基因组学，长片段DNA植物基因组学，及在法庭科学等方面应用，指出了高速PCR技术为各项基因研究可能带来的新的进步。

摘　要　 建立了甲苯二次萃取-直接测汞的方法对生物样品中的甲基汞进行分析测定．样品经氢溴酸水解、甲苯萃取、Ｌ-半胱氨酸反萃取等前处理，应用ＤＭＡ-８０直接测汞仪测定．对甲基汞鱼肉标准物质（ＧＢＷ１００２９）和贻贝标准物质（ＳＲＭ２９７６）的实测结果显示，测定值与标准参考值的相对误差小于４％，测定值的相对标准偏差小于２％，甲基汞的检出限为０．６４ｎｇ·ｇ－１，甲苯二次萃取的萃取率在９５％—９９％之间．该方法具有较高的准确度和精密度，适于鱼、贝等生物体中甲基汞的测定，并可将总汞和甲基汞的测定在一台仪器上完成． 关键词　甲基汞，海产品，甲苯萃取，直接测汞法．

　PCR技术是现代分子生物学最基础的技术之一，在实现扩增效果的同时如何缩短PCR的实验时间成为实验工作者亟待解决的问题。本文从高速PCR的概念入手，阐述了实现高速PCR仪器技术的原理、优势，及可能的应用。指出了高速PCR技术的核心在于，不仅要提高PCR 仪器的升降温速率，更重要的是提高PCR样品温度与金属样品槽的一致性，从而达到缩短变性，退火，延伸等停留时间的目的，进而大大的缩短PCR时间。同时也指出，高速PCR对提高反应特异性带来的好处等。

把聚左旋谷氨酸涂在玻璃基底上（ 右 图），表观和实际Zeta电位值在PH2到PH6之 间一致。在更高的PH值，表观Zeta电位的数值 低于实际值。这表明PGA涂层溶胀，离子穿透 PGS涂层形成表面电导率。

SAXS是一种快速、没有破坏性的测试溶液中蛋白质结构的方法。在溶液中蛋白质处于其天然态。从溶液中蛋白质颗粒的质量可以快速判断其聚集状态，同时它作为质量控制参数也可以认定溶液中的蛋白质是否处于我们不欢迎的变性或聚集状态。而且， SAXS能够确定蛋白质在其自然环境中的低聚状态。即使我们可以从晶体学数据中得到蛋白质的高分辨结构，但我们仍无法获知蛋白质在其自然状态下是单体还是低聚体。

从不同样品只不过分离细菌

NADPH oxidase (NOX2) is a multisubunit membrane-bound enzyme complex that, upon assembly in activated cells, catalyses the reduction of free oxygen to its superoxide anion, which further leads to reactive oxygen species (ROS) that are toxic to invading pathogens, for example, the fungus Aspergillus fumigatus. Polymorphonuclear cells (PMNs) employ both nonoxidative and oxidative mechanisms to clear this fungus from the lung. The oxidative mechanisms mainly depend on the proper assembly and function of NOX2. We identified for the first time the NAD(P)H-dependent enzymes involved in such oxidative mechanisms by means of biexponentialNAD(P)H-fluorescence lifetime imaging (FLIM). A specific fluorescence lifetime of 3670±140 picoseconds as compared to 1870 picoseconds for NAD(P)H bound to mitochondrial enzymes could be associated with NADPH bound to oxidative enzymes in activated PMNs. Due to its predominance in PMNs and due to the use of selective activators and inhibitors, we strongly believe that this specific lifetime mainly originates from NOX2. Our experiments also revealed the high site specificity of the NOX2 assembly and, thus, of the ROS production as well as the dynamic nature of these phenomena. On the example of NADPH oxidase, we demonstrate the potential of NAD(P)H-based FLIM in selectively investigating enzymes during their cellular function.

Zooplankton feed in either of three ways: they generate a feeding current, cruise through the water, or they are ambush feeders. Each mode generates different hydrodynamic disturbances and hence exposes the grazers differently to mechanosensory predators. Ambush feeders sink slowly and therefore perform occasional upward repositioning jumps. We quantified the fluid disturbance generated by repositioning jumps in a mm-sized copepod (Re ~ 40). The kick of the swimming legs generates a viscous vortex ring in the wake; another ring of similar intensity but opposite rotation is formed around the decelerating copepod. A simple analytical model, that of an impulsive point force, properly describes the observed flow field as a function of the momentum of the copepod, including the translation of the vortex and its spatial extension and temporal decay. We show that the time-averaged fluid signal and the consequent predation risk is much less for an ambush feeding than a cruising or hovering copepod for small individuals, while the reverse is true for individuals larger than about 1 mm. This makes inefficient ambush feeding feasible in small copepods and is consistent with the observation that ambush feeding copepods in the ocean are all small, while larger species invariably use hovering or cruising feeding strategies.