The widespread use of pesticides can adversely affec thuman health and the environment . Allethrin is a synthetic pesticide belonging to the pyrethroid family. Allethrin causes paralysis and death of insects by targeting their nervous system . Mosquitoes , flies, cockroaches , and ants are all effectively controlled by it. Several formulations of allethrin are available for domestic use , such as sprays , coils , and heated mats. Allethrin's chemical structure is like that of pyrethrins found in chrysanthemums . However , allethrin is much more stable and has a longer residual effect than natural pyrethrins . The long-term stability of allethrin makes it a more effective insecticide but relatively more persistent in the environment.ConclusionSynthetic pyrethroids are among the most challenging chromatographic separations because of their large number of isomers. Therefore, a C18 column, a shape-selective achiral column, and a derivatized cyclodextrin column were employed to analyze the isomeric ratios. JASCO’s PDA detection, followed by circular dichroism detection, allows researchers to conveniently investigate isomeric ratios of allethrin using 1D HPLC systems, all in reversed-phase using ACN/Water mobile phases.