固相萃取法分析非离子表面活性剂

非离子表面活性剂含有亲水基团，当溶解在水中时不会离子化，并且具有不受水硬度或电解质影响的化学性质。非离子表面活性剂可以与几乎任何其他类型的表面活性剂一起使用。近年来，非离子表面活性剂的使用量急剧增加，因为它们具有广泛的性能，如易用性、高去污力、优异的乳化/分散性和低刺激性。2004年制定的水质标准规定，非离子表面活性剂的浓度必须小于0.02 mg/L。已建立的常规分析方法采用固相萃取-吸收分光光度法，其中吸光度是由非离子表面活性剂Co（II）与着色剂4-（2-pyridylazo）间苯二酚（PAR）反应形成的络合物的吸光度。2012年，一种基于SPE-HPLC的新方法被添加到非离子表面活性剂的水质标准中。水质标准规定，非离子表面活性剂的测量应至少对可接受浓度限值的十分之一敏感，所需的变异系数应优于20%。Application Note 2/3Non-ionic Surfactant Analysis by Solid-phase Extraction- High-performanceLiquid Chromatography (SPE-HPLC) UAS.C O Non-ionic Surfactant Analysis by Solid-phase Extraction- High-performance Liquid Chromatography [SPE-HPLC] Introduction A non-ionic surfactant contai n s a hydrophilic group which does not ionize when dissolved in water and has chemical properties making it unaffected by water hardness or electrolytes. A non-ionic surfactant can be used together with virtually any other type of surfactant. The use o f non-ion i c surfactants has increased dramatical l y i n recent years due to t he wide range of properties they offer such as ease of use， high detergency， excellent emulsif i cation/dispersibil i ty and low st i mulation. SPE-HPLC， Non-ionic surfactant ， Heptaoxyethylene dodecyl Application Note Experimenta l Conditions Equipment Pump： PU-2080 Degasser： DG-2080-53 Column Oven： CO-2065 Autosampler： AS-2057 Detector： UV-2070 Column： YMC-Triart C18 (4.6 mml.D. x150 mmL， 5 um) Eluent： 10 mM Sodium tetraborate/Methanol (62/38) Flow Rate： 1.0 mL/min Column Temp： 40℃ Wavelength： 510nm Injection Volume： 20 pL Standard Sample： Heptaoxyethylene dodecyl ether Results Fig . 1 Flowchart of sample preparation. Fi g.1. Flowchart for Preparation of Sample Added with Non-ionic Surfactant F i g. 2. Chromatogram of Sample Added with Non -i on i c Su r factant*(n =6)1： Heptaoxyethylene dodecyl ethe r (0.002 mg/L)*Sample preparation is described on Fi g . 1. PeakArea 1 28974 2 22078 3 28057 4 25492 5 19989 6 29604 AVE 25699 SD 3931.7 C.V.% 15.3 Fig. 3 linearity of sample with added non-ionic surfactant . Four standard samples were measured with concentrations ranging from 0.002 to 0.01 mg/L as specified i n the of f icial analytical method， linear i ty was R2=0.985.