非离子表面活性剂的HPLC-ELSD分析

表面活性剂是商用洗涤剂的主要成分，由于具有洗涤性能、吸湿性、渗透性、溶解性、分散性、润滑性、抗静电性、杀菌性和防锈性等多种性能，被广泛应用于各种工业和制药领域。非离子表面活性剂具有亲水基团，即使溶解在水中也不会离子化。由于它几乎不受水硬度和电解质的影响，因此可以与所有其他表面活性剂一起使用。鉴于其性质，非离子表面活性剂的使用量急剧增加，并指出了其对环境的影响。自2003年以来，非离子表面活性剂一直是《水质控制标准》的检测项目。这一次，使用具有二氧化硅NH2柱的ELSD检测器在HILIC模式下测量和分析作为非离子表面活性剂样品的Triton X-100和聚乙二醇400。Application Note 2/3Application NoteAnalysis of Nonionic Surfactants by HPLC-ELSD Analysis of Nonionic Surfactants by H PLC-ELSD Surfactants are well known as a main constituent of commercial detergent a nd is widely used in various in d ustrial and pharmaceutical f ield s due to various capabilities such as washing performance， moisture absorbency， osmosis， solubility， dispersibility，lubr i c i ty， antistatic ability ， bactericidal property and anti -rust property. Nonionic surfactants have hydrophilic groups which are not ionized even when dissolved in water . Since it is hardly aff e cted by water hardness and electrolytes， it can be used together with all other surfactants. Consider i ng its nature ， the usage of nonionic surfactants have i ncre a sed dram a tically and its affect on the environment has been pointed out. Since 2003，nonionic surfactants have been the test i tem for Water Quality Control Standards. This time ， Triton X-100 and Polyethylene Glycol 400 as no n ionic surfactant samples were measured and analyzed using an ELSD detector with Sil i ca NH2 column in HILIC mode. Keywords ： Nonionic surfactants， Triton X-100，PEG400， HILIC， Silica NH2 column， ELSD PU-2089 Experimental Equipment： Conditions： Pump： PU-2089 Autosampler： AS-2057 Column oven： Detector： ELS-2040 Column： Finepak SIL NH2-5 (4.6 mmlDx250 mmL) Eluent A： Acetonitrile Eluent B： Acetonitrile/0.1% Acetic acid (90/10) Gradient condition： Triton X-100； ，(A/B)， 0 min (90/10) 16 min (2/98) ，26 min (2/98) ，26.1min (90/10) 1 cycle； 40 min，PEG400；(A/B) (50/50) 1.0 mL/min Flow rate： Column temp.： 30°C ELSD condition： Nebulizer temp.； 30℃， Evaporator temp.； 60℃， Gas flow rate； 1.6 SLM Injection volume： 10 uL Standard sample： Triton X-100， PEG400 30 mg/mL eachin Acetonitrile R esults and Discussion Figu r e 1 a n d fi gur e 2 sho w t he c hr o ma t og ra m s of Trit o n X -100 a n d PE G 400，res p e cti vely. F i gure 1 C hromatogram o f T r ito n X -100. A p plicat i o n L i br a r y ： htt p ：//www.j as co i n c .c o m /ap p l i c a ti o n s Application Note