纳谱分析NanoChrom纳谱分析技术(苏州)有限公司NanoChrom Technologies (Suzhou) Co., Ltdwww.nanochrom.com 标准蛋白的分离 Time [min] Column: BioCore HIC-Butyl, 5 pmDimension: 4.6x100 mm Mobile phase A: 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0 Mobile phase B: 100 mM phosphate buffer, pH7.0 Gradient:t (min)%A%B-101000010001000010020100 Flow rate: 1.0 mL/min Temperature: 30℃ Injection: 10 pL Detection: UV 280 nm Sample: (~1mg/mL each in mobile phase A) 1. Ribonuclease A 2. Myoglobin 3. Lysozyme 4.a-Chymotrypsinogen A Column: BioCore HIC-Butyl, 5 μmDimension: 4.6x100 mmMobile phase A: 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0Mobile phase B: 100 mM phosphate buffer, pH7.0Mobile phase C: IsopropanolGradient: t (min) %A %B %C -10 75 25 0 0 75 25 0 1 75 25 0 15 0 75 25 20 0 75 25Flow rate: 1.0 mL/minTemperature: 30 ℃Injection: 10 μLDetection: UV 280 nmSample: Cysteine conjugated ADC (~1 mg/mL in mobile phase A)