纳谱分析NanoChrom纳谱分析技术(苏州)有限公司NanoChrom Technologies (Suzhou) Co., Ltdwww.nanochrom.com IgG4单抗的电荷异质体分离 Time [min] Column: BioCore WCX, 10 um Dimension: 4.6×150mm Mobile phase A: 20 mM PBS, pH6.5 Mobile phase B: 20 mM PBS, pH6.5+300 mM NaCl Gradient: L A B-15 50 505 2020.1 0 10023 0 100 Flow rate: 1 mL/min Temperature: 30 ℃ Injection: 25 pL Detection: UV 280 nm Sample: lgG44(1mL/min in water) Column: BioCore WCX, 10 μmDimension: 4.6×150mmMobile phase A: 20 mM PBS, pH6.5Mobile phase B: 20 mM PBS, pH6.5 + 300 mM NaClGradient: t A B -15 95 5 0 95 5 0.1 95 5 20 80 20 20.1 0 100 23 0 100Flow rate: 1 mL/minTemperature: 30 ℃Injection: 25 μLDetection: UV 280 nmSample: IgG4 (1mL/min in water)