Analytical Equipment一二一 DETERMINATION OF METHIONINE HYDROXY ANALOGUEIN FODDER ADDITIVES Lumex Method M 04-83-2014 INTRODUCTION The method is used for the determination ofthe mass ffraction of methionine hydroxy analogue(DL-2-hydroxy-4-(methylthio)butyric acid (HMTBa) and its calcium salt) in fodder additives by capillaryelectrophoresis. Currently, fodder additives containing HMTBa or its calcium salt, are presented on the market under suchbrand names as MHA, Alimet@, Rhodimet@,and others. For the determination of free methionine in fodder additives use the method M 04-63-2016 (LumexInstruments set, order No 0300002780). MEASUREMENT METHOD The measurement method is based on capillary zone electrophoresis with direct UV detection at thewavelength of 200 nm. MEASUREMENT RANGE The measurement range for HMTBa is 60-100%. If necessary, a result of analysis may be expressed asmethionine or calcium salt of HMTBa. ADVANTAGES OF CE METHOD CE method for the determination of HMTBa has a lot of advantages compared with other analytical methodsused for this purpose, such as HPLC,potentiometry, complexometry: ● The HPLC column is not required, so the cost of the analysis drops dramatically compared withHPLC. Direct determination of the main analyte (HMTBa) in contrast to the indirect determination ofcalcium (complexometry) and/ or total organic sulfur (potentiometry) content. Analysis time using CAPEL CE system is less than 5 minutes. EQUIPMENT AND REAGENTS The CAPEL capillary electrophoresis system is used in measurements. Data acquisition, collection,processing and output are performed using a personal computer running under WINDOWS@ XP/7/8/10operating system with installed dedicated software package ELFORUN. 1Lumex Instruments set, order No 0300001888. EXAMPLES OF REAL ANALYSES The contents on this paper are subject to change without notice.To get more specific information, please contact the representative by sales@lumexinstruments.com www.lumexinstruments.ccm INTRODUCTIONThe method is used for the determination of the mass fraction of methionine hydroxy analogue (DL-2-hydroxy-4-(methylthio)butyric acid (HMTBa) and its calcium salt) in fodder additives by capillary electrophoresis.Currently, fodder additives containing HMTBa or its calcium salt, are presented on the market under such brand names as MHA®, Alimet®, Rhodimet®, and others.For the determination of free methionine in fodder additives use the method М 04-63-2016 (Lumex Instruments set, order No 0300002780).MEASUREMENT METHODThe measurement method is based on capillary zone electrophoresis with direct UV detection at the wavelength of 200 nm.MEASUREMENT RANGEThe measurement range for HMTBa is 60–100%. If necessary, a result of analysis may be expressed as methionine or calcium salt of HMTBa.ADVANTAGES OF CE METHODCE method for the determination of HMTBa has a lot of advantages compared with other analytical methods used for this purpose, such as HPLC, potentiometry, complexometry:· The HPLC column is not required, so the cost of the analysis drops dramatically compared with HPLC.· Direct determination of the main analyte (HMTBa) in contrast to the indirect determination of calcium (complexometry) and/ or total organic sulfur (potentiometry) content.· Analysis time using CAPEL CE system is less than 5 minutes.