白兰地和白兰地酒中香兰素、芥子碱、松柏醇和丁香醛检测方案(毛细管电泳仪)

HIGH PERFORMANCECAPILLARYELECTROPHORESIS SYSTEMWWW.LUMEX.BIZ DETERMINATION OF VANILLIN， SINAPIC， CONIFERYL， AND SYRINGIC .ALDEHYDES IN BRANDIES AND BRANDY SPIRITS LUMEX Method M 04-53 (2008) INTRODUCTION The presence or absence of aromatic aldehydes (vanillin， sinapic， coniferyl and syringic aldehydes) in asample and also their weight content and proportion allows determination of authenticity and quality ofbrandies and brandy spirits， and revealing a counterfeit. Together with aromatic aldehydes， it is possible todetermine phenolcarbolic acids， such as sinapic， syringic， ferulic， salicylic， cumaric， vanillic， ellagic，p-hydroxybenzoic， caffeic， gallic， protocatechuic. Identification and weight content of these acids is anadditional criterion of the spirit authenticity. MEASUREMENTMETHOD Capillary electrophoresis method allows simultaneous determination of all compounds by the method ofcapillary zone electrophoresis in a borate buffer with direct detection at the 373 nm wavelength. MEASUREMENT RANGE Ranges of measurable concentrations for analyzed aldehydes are 0.2-50 mg/L EQUIPMENT AND REAGENTS The“CAPEL-105/105M" capillary electrophoresis system is used in measurements. Data acquisition， collection， processing and output are performed using a personal computer running under“WINDOWS 2000/XP”operating system with installed dedicated software package for acquisition andprocessing of chromatography data. All reagents must be of analytical grade or higher. EXAMPLE OF A REAL ANALYSIS Buffer： borate Capillary： The content of this application note is subject to change without notice. 芳香醛（香兰素、芥子碱、松柏醛和丁香醛）在白兰地酒中的存在与否，以及它们的质量含量和比例影响了白兰地和白兰地酒的真假，并以此判断是否为假酒。同芳香醛一起，可以测定酚碳酸根，如芥子酸、丁香酸、阿魏酸、水杨酸、多糖、香兰素、鞣花剂、对羟基苯甲酸、咖啡酸、没食子酸、原儿茶酸等。这些酸的鉴定和质量含量是真假酒鉴别的重要标志。毛细管电泳法可以在373 nm波长下直接检测在硼酸盐缓冲液中的所有化合物。