γ-氨基丁酸中γ-氨基丁酸含量检测方案(氨基酸分析仪)

SAMPLE 20 uL of std. soln. (2 nmol/20 uL each) PRESSURE PACKINGMATERIAL #2622 [HITACHI TEMPERATURE 52-57°℃ COLUMN SIZE for separation 4.6 mm I.D. ×60 mm [#2622]for ammonia trap 4.6 mm I.D.×40 mm [#2650L] SEPARATION METHOD Ion exchange DETECTOR VIS 570 nm. 440 nm ELUENT L-8500 PH-Kit INSTRUMENTS L-8900 (Amino Acid Analyzer) FLOW RATE 0.4mL/min NOTE Reaction reagent = ninhydrin reagent， Flow rate=0.35 mL/min Reaction unit temperature=135℃ This analysis was conducted using the buffer and column for L-8900 protein hydrolysate analysis method and modified gradient program. KEY WORDS Amino Acid， r-Amino-n-butyric Acid， GABA， Ornithine， Protein HydrolysateAnalysis Method， Ninhydrin Method， UV-VIS Spectrometry，L-8900， Ion Exchange High Performance Liquid Chromatograph(HPLC) Sheet No. LC050080-01A The molecular weight table of Amino acids Abbreviations， compounds， molecular weights of amino acids are shown. Abbrev Compound MW Asp Aspartic acid 133.1 Thr Threonine 119.1 Ser Serine 105.1 Glu Glutamic acid 147.1 Pro Proline 115.1 Gly Glycine 75.1 Ala Alanine 89.1 Cys Cystine 240.3 Val Valine 117.1 Met Methionine 149.2 Ile Isoleucine 131.2 Leu Leucine 131.2 Tyr Tyrosine 181.2 Phe Phenylalanine 165.2 g-ABA r-Amino-n-butyric acid 103.1 Orn Ornithine 132.2 Lys Lysine 146.2 NH： Ammonia 17.0 His Histidine 155.2 Arg Arginine 174.2 KEY WORDS Amino Acid， r-Amino-n-butyric Acid， GABA， Ornithine， Protein HydrolysateAnalysis Method， Ninhydrin Method， UV-VIS Spectrometry，L-8900， Ion Exchange High Performance Liquid Chromatograph(HPLC) Sheet No. LC050080-02A