植物中荧光检测方案(植物荧光成像)

EcoTech易科泰生态技术科泰 生态 技术 有 限 公 司Ecotech Science and Technology Ltd. 易 植物表型组学研究技术(一)——FluorCam叶绿素荧光成像技术 FluorCam 叶绿素荧光成像技术 Rousseau等 (High throughput quantitative phenotyping of plant resistance using chlorophyll fluorescence image analysis. Plant Methods， 2013，9：17)，利用FluorCam开放式叶绿素荧光成像系统作为高通量表型分析平台，采用图像阈值分割等分析方法，对植物病原体感染进行了定量分析检测，根据Fv/Fm将感染分为不同阶段/等级，特别是可以将用其它方法难以分辨出来的感染前期加以分辨，并对5个品种的菜豆对普通细菌性疫病的抗性进行了定量分析评价。 A B Figure 1 Expert-based thresholds allow the segmentation of various stages of the symptom development. Two weeks-old bean plantscv. Flavert were inoculated with either Xff CFBP4834-R(1.10° CFU ml-') or mock. This leaflet inoculated with Xff CFBP4834-R was sampled onbean P.vulgaris cv. Flavert at 11 dai. A： visible image obtained by scanning. Necrosis is clearly visible on the left marge of the leaflet surroundedby wilted tissues. B： F/Fm image obtained by chlorophyll fluorescence imaging. The three stages of the symptom development，i.e. necrotic，wilted and impacted tissues， were segmented respectively with the thresholds 0.25 ≤F/Fm， 0.25

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