HPLC DATA 'SHEET CH3 Column :YMC CHIRAL Cellulose-SB (5pm)250X4.6mml.D.Eluent : acetonitrile/20 mM phosphoric acid (50/50)Flow rate : 1.0 mL/minTemperature :25℃Detection : UV at 254 nmInjection : 2 uL (200 ug/mL) 采用CHIRAL ART Cellulose-SB手性柱分离华法林手性异构体,分离度达7.3。