HPLC DATA'SHEET E下Glimepiride U101117A A) Standard solution*(0.005 mg/mL Glimepiride) 0 5 10 15 20 25 min * All standard and sample solutions were prepared from Glimepiride supplied as a reagent for laboratory use. Column :YMC-Pack Diol-NP (5 um, 12 nm) 150X3.0mml.D. Eluent : heptane/2-propanol/acetic acid (900/100/1) Flow rate :0.5 mL/min Temperature :25℃ Detection : UV at 228 nm Injection :10uL 色谱条件见谱图:符合日本药典、美国药典要求。