盐酸吡格列酮中含量/有关物质检测方案(液相色谱柱)

HPLC DATASHEET 匕才夕夕少少墟酸墟Pioglitazone hydrochloride U100927A A) Assay：Sample solution*(0.05 mg/mL Pioglitazone HCI， 0.013 mg/mL Benzophenone] 5 H ·HCI Pioglitazone hydrochloride B) Related substances/Organic impurities： Sample solution*(0.2 mg/mL Pioglitazone HCI) 2 0 Benzophenone *Sample solutions were prepared from Pioglitazone HCI supplied as a reagent for laboratory use. Column ： YMC-Triart C18 (5 um， 12 nm) 150X4.6mml.D. Eluent ： acetonitrile/100mM CH3COONHa/acetic acid (25/25/1) Flow rate ：0.8 mL/min Temperature ：25℃ Detection ： UV at 269 nm Injection ：A)20 uL B) 40 pL- (The Japanese Pharmacopoeia 15th 2nd supplement， The United States Pharmacopoeia 34th) 色谱条件见谱图