The Agilent Thermal Equilibration Device Increases Resolution for Oligonucleotide Analysis

Due to advances in oligonucleotide synthesis and modification, modern DNA‑ or RNA‑based biopharmaceuticals can make characterization more demanding because of their increased complexity. This application note demonstrates that the Agilent Thermal Equilibration Device (TED) combined with the biocompatible Agilent 1290 Infinity II Bio LC is an excellent setup for in‑depth analysis of oligonucleotides. Using the TED with a high‑temperature LC method, the peak resolution and analysis speed can be significantly increased. Product and processimpurities can be separated and analyzed. These results show that oligonucleotide analysis can significantly benefit from the TED.