Analytical EquipmentHIGH PERFORMANCECAPILLARY ELECTROPHORESISSYSTEMwww.lumexinstruments.ccm DETERMINATION OF ASCORBIC ACID AND ITS SALTS IN FOOD ADDITIVES AND FODDER ADDITIVES Lumex Method M 04-86-2016 INTRODUCTION The method is used for the determination of the mass fraction of ascorbic acid and its salts in food additivesand fodder additives (feed additives). The present method does not allow the determination of ascorbic acid in premixes. For the determination of acetic, benzoic, butyric, citric, formic, fumaric, lactic, malic, oxalic, propionic, sorbic,and succinic acids in fodder additives and butyric acid in silage and haylage use the method M 04-74-2012(Lumex Instruments set, order No 0300001897). MEASUREMENTMETHOD The measurement method is based on capillary zone electrophoresis with direct UV detection at thewavelength of 254 nm. MEASUREMENT RANGE The measurement range for ascorbic acid is 5-100% EQUIPMENT AND REAGENTS The CAPEL capillary electrophoresis system is used in measurements. Data acquisition, collection,processing and output are performed using a personal computer running under WINDOWS@XP/7/8/10operating system with installed dedicated software package ELFORUN. Lumex Instruments set, order No 0300002717. EXAMPLES OF REAL ANALYSES BGE: borate Capillary: Lefi/ Ltot 40/50 cm, ID 50 um Injection: 150mbar x sec Voltage: + 20 kV Temperature:+20°c Detection: The contents on this paper are subject to change without notice.To get more specific information, please contact the representative by sales@lumexinstruments.com DETERMINATION OF ASCORBIC ACID AND ITS SALTSIN FOOD ADDITIVES AND FODDER ADDITIVESINTRODUCTIONThe method is used for the determination of the mass fraction of ascorbic acid and its salts in food additives and fodder additives (feed additives).The present method does not allow the determination of ascorbic acid in premixes.For the determination of acetic, benzoic, butyric, citric, formic, fumaric, lactic, malic, oxalic, propionic, sorbic, and succinic acids in fodder additives and butyric acid in silage and haylage use the method М 04-74-2012 (Lumex Instruments set, order No 0300001897).MEASUREMENT METHODThe measurement method is based on capillary zone electrophoresis with direct UV detection at the wavelength of 254 nm.MEASUREMENT RANGEThe measurement range for ascorbic acid is 5–100%.EQUIPMENT AND REAGENTSThe CAPEL capillary electrophoresis system is used in measurements. Data acquisition, collection, processing and output are performed using a personal computer running under WINDOWS® XP/7/8/10 operating system with installed dedicated software package ELFORUN.Lumex Instruments set, order No 0300002717.EXAMPLES OF REAL ANALYSESBGE: borateCapillary: Leff/ Ltot 40/50 cm, ID 50 µmInjection: 150 mbar x sec Voltage: + 20 kV Temperature: + 20 ОС Detection: 254 nm