软饮料中咖啡因、抗坏血酸、防腐剂（苯甲酸、山梨酸）、人工甜味剂（安赛蜜、糖精钠）检测方案(毛细管电泳仪)

Analytical Equipment DETERMINATION OF CAFFEINE， ASCORBIC ACID， PRESERVATIVES(BENZOIC ACID， SORBIC ACID， AND THEIR SALTS)， AND ARTIFICIALSWEETENERS (ACESULFAME K， SACCHARIN) IN SOFT AND STRONG DRINKS Lumex Method M 04-51-2008 INTRODUCTION The method is used for the determination of the mass concentration of caffeine，ascorbic acid and its salts，sorbic acid and its salts，benzoic acid， and its salts， acesulfame K， saccharin and its salts in beverages.The method can be applied for all types of non-alcoholic and alcoholic beverages including sport andenergetic drinks， juices， beer and beer products， wines， brandy and spirits， liquors， and vodka. MEASUREMENTMETHOD The micellar electrokinetic chromatography (MEKC) allows separation of neutral and ionic forms of analyzedcomponents. The components are detected by intrinsic absorption at awavelength of 254 nm. MEASUREMENT RANGE Measurement ranges of analyzed components are presented in the table below. Compound CSFA code E number Determined form Measurement range*，mg/L Caffeine 一 一 Caffeine 10-1000 Sorbic acid INS 200 E 200 Sorbic acid Sodium sorbate Potassium sorbate Calcium sorbate INS 201 E201 INS 202 Calcium sorbate INS 203 E 203 Benzoic acid Sodium benzoate Potassium benzoate Calcium benzoate INS 210 E 210 Benzoic acid Sodium benzoate INS 211 E211 INS 213 INS 300 E 212E 213 E 300 Ascorbic acid Sodium ascorbate E 301E 302 Calcium ascorbate Potassium ascorbate INS 302 Acesulfame K INS 303 INS 950 E 303 E 950 Acesulfame K Saccharin Calcium saccharin Potassium saccharinSodium saccharin INS 954(i) Sodium saccharin INS 954(ii) INS 954(iii) INS 954(iv) E 954(iv) *FFor every mentioned form of the food additive. The present method does not allow separation of such food additives as E 200 -E 203， E210- E 213，E 300 - E 303， and E 954. Other sweeteners (aspartame， cyclamate) synthetic food dyes， vitamins B， andvanillin do not hinder the analysis if added in concentrations， typical for the analyzed drinks. EQUIPMENT AND REAGENTS The CAPEL capillary electrophoresis system is used in measurements. Data acquisition， collection，processing and output are performed using a personal computer running under WINDOWS XP/7/8/10operating system with installed dedicated software package ELFORUN. Lumex Instruments complex set， order No 0300002054. ( '-National Standard GOST R 53193-2008， SM GOST R 53193：2012 (Moldova). ) EXAMPLES OF REAL ANALYSES BGE： borate， with SDS Capillary：-ef/ -tot 50/60 cm， ID 75 pmInjection：150 mbar x secVoltage：+25 kVTemperature：+20°cDetection：254nm Sample： test mixture (30 mg/L of eachcompound) 1-caffeine 2 - ascorbic acid 3- sorbic acid 4 -benzoic acid 5 - sodium saccharin 6-acesulfame K Sample： soft drink， undilutedMeasurement results： 1- caffeine (90 mg/L) 2- benzoic acid (120 mg/L) 3-acesulfame K (130 mg/L) Sample： energy drink， fivefold diluted Measurement results： 1 - caffeine (325 mg/L) 2 - ascorbic acid (20 mg/L) 3-vanillin 4 - sorbic acid (220 mg/L) The contents on this paper are subject to change without notice.To get more specific information， please contact the representative by sales@lumexinstruments.com www.lumexinstruments.ccm INTRODUCTIONThe method1 is used for the determination of the mass concentration of caffeine, ascorbic acid and its salts, sorbic acid and its salts, benzoic acid, and its salts, acesulfame K, saccharin and its salts in beverages.The method can be applied for all types of non-alcoholic and alcoholic beverages including sport and energetic drinks, juices, beer and beer products, wines, brandy and spirits, liquors, and vodka.MEASUREMENT METHODThe micellar electrokinetic chromatography (MEKC) allows separation of neutral and ionic forms of analyzed components. The components are detected by intrinsic absorption at a wavelength of 254 nm.MEASUREMENT RANGEMeasurement ranges of analyzed components are presented in the table below. CompoundCSFA codeЕ numberDetermined formMeasurement range*, mg/LCaffeine––Caffeine         10–1000Sorbic acidINS 200E 200  Sorbic acidSodium sorbateINS 201E 201Potassium sorbateINS 202E 202Calcium sorbateINS 203E 203Benzoic acidINS 210E 210Sodium benzoatePotassium benzoateINS 211INS 212E 211E 212Benzoic acidCalcium benzoateINS 213E 213Ascorbic acidINS 300E 300  Ascorbic acidSodium ascorbateINS 301E 301Calcium ascorbateINS 302E 302Potassium ascorbateINS 303E 303Acesulfame KINS 950E 950Acesulfame KSaccharinINS 954(i)E 954(i)  Sodium saccharinCalcium saccharinINS 954(ii)E 954(ii)Potassium saccharinINS 954(iii)E 954(iii)Sodium saccharinINS 954(iv)E 954(iv)* For every mentioned form of the food additive.The present method does  not allow separation of such food additives as E 200 – E 203, E210 – E 213,         E 300 – E 303, and E 954. Other sweeteners (aspartame, cyclamate) synthetic food dyes, vitamins B, and vanillin do not hinder the analysis if added in concentrations, typical for the analyzed drinks.EQUIPMENT AND REAGENTSThe CAPEL capillary electrophoresis system is used in measurements. Data  acquisition,  collection,  processing and output are performed using a personal computer running under WINDOWS® XP/7/8/10 operating system with installed dedicated software package ELFORUN.Lumex Instruments complex set, order No 0300002054.