软饮料和酒精饮料中合成染料（柠檬黄、日落黄、苋菜红、胭脂红、赤藓红、靛蓝、亮蓝）检测方案(毛细管电泳仪)

HIGH PERFORMANCECAPILLARYELECTROPHORESIS SYSTEM寸山6WWW.LUMEX.BIZ IDENTIFICATION AND DETERMINATIONOF SYNTHETIC DYES IN SOFT ANDALCOHOLIC DRINKS LUMEX Method M 04-48 (2007) INTRODUCTION The method allows identification and determination of synthetic dyes in samples of soft and alcoholic drinksby capillary electrophoresis. MEASUREMENT METHOD The Capillary Electrophoresis (CE) method for the determination of synthetic dyes is based on theirdifferential migration in a narrow quartz capillary under the influence of the applied electric field. Identificationand quantitative determination of dyes is performed by measuring the UV absorbance at 254 nm (for“CAPEL-103PT/104T"systems) or 215 nm (for"CAPEL-105/105M" systems) wavelength. MEASUREMENT RANGES Name (C. I.) E number Measurement range，mg/L Tartrazine (C.I. 19140) E 102 1.0-250 Sunset Yellow FCF (C. I. 15985) E 110 1.0-250 Carmoisine (C. I.14720) E 122 1.0-250 Amaranth (C. I. 16185) E 123 1.0-50 Ponceau 4R (C. I. 16255) E 124 1.0-250 Erythrosine (C. I. 45430) E 127 1.0-50 Red 2G (C. I. 18050) E 128 1.0-50 Allura Red AC (C. I. 16035) E 129 1.0-250 Patent Blue V (C. 1. 42051) E 131 1.0-250 Indigotine (C. I. 73015) E 132 1.0-250 Brilliant Blue FCF (C. 1.42090) E 133 5.0-250 Green S (C. I. 44090) E 142 1.0-250 Brilliant Black BN (C. I. 28440) E151 1.0-250 If concentration of one or several dyes is higher than the upper limit of the range， it is possible to dilute thesample by distilled water. EQUIPMENT AND REAGENTS The“CAPEL-103RT/104T/105/105M” capillary electrophoresis system with high-voltage positive polarity isused in measurements. Data acquisition， collection， processing and output are performed using a personal computer running under"WINDOWS 2000/XP”operating system with installed dedicated software package for acquisition andprocessing of chromatography data. All reagents must be of analytical grade or higher. The contents on this paper are subject to change without notice. 人工合成色素广泛用作饮料、化妆品等的着色剂,由于它具有一定毒性,因此对其进行分析测定具有极其重要的意义。该方法允许通过毛细管电泳对软饮料和酒精饮料样品中的合成染料进行鉴定和测定。毛细管电泳法测定合成染料是基于它们在电场作用下在窄石英毛细管中的微分迁移。染料的鉴定和定量测定是通过测量在波长254 nm处或215nm处的紫外吸光度进行测定的。